Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma
Proliferating Cell Nuclear Antigen in Premalignancy and Oral Squamous Cell Carcinoma
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DOI：
10.7860/jcdr/2015/12645.6094
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发表时间：
2015-06-01
影响因子：
0.2
通讯作者：
Sundar, Mural
中科院分区：
文献类型：
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作者：
Poosarla, Chandrashekar;Ramesh, Maya;Sundar, Mural
前言：癌症的病因是多因素的，是一个多步骤的过程，包括启动、促进和肿瘤进展。细胞增殖是判断恶性病变生物学侵袭性的重要指标之一。增殖异常可能是判断多种恶性肿瘤潜在预后的重要指标。目的：本研究旨在探讨增殖细胞核抗原的表达作为判断口腔癌前病变和鳞癌临床侵袭性的指标。材料和方法：50例口腔癌前病变和鳞癌患者均经组织病理学检查证实。包括正常口腔黏膜(10例)、不典型增生(10例)和口腔鳞癌分级(30例)，年龄在40-60岁之间。从每个块中制备4微米厚的切片，放置在涂有多聚L赖氨酸的玻片上。用单抗(PC10)进行免疫组织化学染色。染色的玻片由一名检查员对细胞计数进行评估。结果：研究组和对照组之间的比较显示出概率值(p值)&t；0.05。从正常口腔鳞癌到口腔鳞状细胞癌的增殖指数显著增加。低分化鳞癌的增殖指数最高，其次是中分化、高分化鳞癌、异型增生和正常口腔黏膜。结论：增殖细胞核抗原指数可以用来评价异型增生和不同级别口腔鳞癌的增殖和侵袭性。
Introduction: Cancer has multifactorial aetiology and is a multistep process involving initiation, promotion and tumour progression. Cellular proliferation is one of the important indicators for the biologic aggressiveness of a malignant lesion. The dysregulated proliferation may be a significant change to determine the potential prognosis of various malignant tumours.Aim: The aim of this study was to evaluate the expression of proliferating cell nuclear antigen (PCNA) as an indicator for clinical aggressiveness in oral premalignancy and squamous cell carcinoma.Materials and Methods: A total of 50 blocks were taken from the Department of Oral Pathology which was diagnosed previously histopathologically. It comprised of normal oral mucosa (10), dysplasia (10) and grades of oral squamous cell carcinoma (30) of patients between the age group of 40-60 years. From each block, sections of 4 micro metre thicknesses were prepared and placed on poly-L lysine coated slides. These sections were immunohistochemically stained with monoclonal proliferating cell antibody (PC10). The stained slides were evaluated by a single examiner for cell count.Results: A comparison between study groups and controls showed a probability value (p-value) < 0.05. Significant increase in the proliferative index from the normal to oral squamous cell carcinoma was noticed. Poorly differentiated squamous cell carcinoma showed maximum proliferative index followed by moderately differentiated, well differentiated squamous cell carcinoma, dysplasia and normal mucosa.Conclusion: Present study concluded that PCNA index can be used to assess the proliferation and aggressiveness in dysplasia and different grades oral squamous cell carcinoma.