The EHI motif in metazoan transcription factors
The EHI motif in metazoan transcription factors
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DOI：
10.1186/1471-2164-6-169
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发表时间：
2005-11-27
期刊：
影响因子：
4.4
通讯作者：
Copley, RR
中科院分区：
文献类型：
--
作者：
Copley, RR
背景资料：Engrailed Homology 1（EH 1）基序是一个小区域，据信在含有同源框和叉头的蛋白质中趋同进化，与果蝇蛋白groucho相互作用（C. elegans unc- 37，人转导蛋白样分裂增强子）。模体的小尺寸使得难以通过计算手段对其进行可靠的识别。我系统地搜索了果蝇C.结果：利用模体识别方法和数据库检索技术，确定了哪些同源框和叉头结构域蛋白也可能具有EH 1模体。我表明，尽管低数据库搜索分数，有一个显着的关联的基序与转录因子的功能。我进一步表明，可能的EH 1基序被发现与T-盒，锌指和Doubletex结构域的组合，以及讨论其他可能的候选协会。我在基底后生动物门中鉴定出了强候选的EH 1基序。结论：候选的EH 1基序与多种转录因子结构域结合存在，表明这些蛋白质具有抑制子功能。EH 1基序的分布暗示了趋同进化，尽管在许多情况下，该基序在两侧向同源物中是保守的。Groucho介导的阻遏作用在双侧体进化之前就已经建立。
Background: The Engrailed Homology 1 (EH1) motif is a small region, believed to have evolved convergently in homeobox and forkhead containing proteins, that interacts with the Drosophila protein groucho (C. elegans unc- 37, Human Transducin- like Enhancers of Split). The small size of the motif makes its reliable identification by computational means difficult. I have systematically searched the predicted proteomes of Drosophila, C. elegans and human for further instances of the motif.Results: Using motif identification methods and database searching techniques, I delimit which homeobox and forkhead domain containing proteins also have likely EH1 motifs. I show that despite low database search scores, there is a significant association of the motif with transcription factor function. I further show that likely EH1 motifs are found in combination with T- Box, Zinc Finger and Doublesex domains as well as discussing other plausible candidate associations. I identify strong candidate EH1 motifs in basal metazoan phyla.Conclusion: Candidate EH1 motifs exist in combination with a variety of transcription factor domains, suggesting that these proteins have repressor functions. The distribution of the EH1 motif is suggestive of convergent evolution, although in many cases, the motif has been conserved throughout bilaterian orthologs. Groucho mediated repression was established prior to the evolution of bilateria.