Low-cost fluctuating-temperature chamber for experimental ecology
Low-cost fluctuating-temperature chamber for experimental ecology
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DOI：
10.1111/2041-210x.12619
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发表时间：
2016-12-01
影响因子：
6.6
通讯作者：
Alford, Ross A.
中科院分区：
文献类型：
--
作者：
Greenspan, Sasha E.;Morris, Wayne;Alford, Ross A.
1.市售的波动温度室大且昂贵。这对努力在实验室中重现自然温度循环的实验生态学家提出了挑战，因为复制研究设计所需的大量商业室购买起来非常昂贵，需要大量的空间并消耗大量的能源。我们开发并验证了一种经济、可编程的温度波动室设计，该设计基于一个相对较小的（23 L）商业制造的恒温室（140美元），并使用定制的用户友好型微控制器（15美元）进行了修改。3.经过一周的试验，这些室可靠地再现了真实世界的波动，（13.1-35.5 ℃）个体青蛙的体温状态，目标温度和实际温度之间接近完美的1：1拟合（y = 1.0036x + 0.1366，R-2 = 0.9977，斜率的95%置信区间= 1.0026，1.0046）。在30天的试验中，他们还可靠地产生了一个更简单的每日波动温度方案（正弦波每24小时在10到25摄氏度之间波动）和一系列恒定温度方案。该设计价格低廉，易于大量组装，能够真正复制甚至高度复杂的许多治疗研究设计。例如，有可能在重复室中同时检查生物体对恒定制度的反应，根据种群所经历的平均值波动的制度，以及精确模拟在任何时间长度上测量的行为或微生境使用不同的特定个体的波动的制度。因此，这些腔室极大地扩展了热生物学和生态学中可用于操纵实验的资源库。
1. Commercially available fluctuating-temperature chambers are large and costly. This poses a challenge to experimental ecologists endeavouring to recreate natural temperature cycles in the laboratory because the large number of commercial chambers required for replicated study designs is prohibitively expensive to purchase, requires a large amount of space and consumes a great deal of energy.2. We developed and validated a design for economical, programmable fluctuating-temperature chambers based on a relatively small (23 L) commercially manufactured constant temperature chamber ($140US) modified with a customized, user-friendly microcontroller ($15US).3. Over a 1-week trial, these chambers reliably reproduced a real-world fluctuating (13.1-35.5 degrees C) body temperature regime of an individual frog, with a near-perfect 1 : 1 fit between target and actual temperatures (y = 1.0036x + 0.1366, R-2 = 0.9977, 95% confidence interval for slope = 1.0026, 1.0046). Over 30-day trials, they also reliably produced a simpler daily fluctuating-temperature scheme (sine wave fluctuating between 10 and 25 degrees C each 24 h) and a range of constant temperature regimes.4. The design is inexpensive and simple to assemble in large numbers, enabling genuine replication of even highly complex, many treatment study designs. For example, it is possible to simultaneously examine in replicate chambers the responses of organisms to constant regimes, regimes that fluctuate following the means experienced by populations and regimes that exactly mimic fluctuations measured over any length of time for particular individuals that differ in behaviour or microhabitat use. These chambers thus vastly expand the pool of resources available for manipulative experiments in thermal biology and ecology.