Interspecies transmission of influenza C virus between humans and pigs
Interspecies transmission of influenza C virus between humans and pigs
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DOI：
10.1016/s0168-1702(96)01427-x
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发表时间：
1997-04-01
期刊：
影响因子：
5
通讯作者：
Nakamura, K
中科院分区：
文献类型：
--
作者：
Kimura, H;Abiko, C;Nakamura, K
本文对1991年1月至1993年2月在日本山形市和仙台市分离的18株人源丙型流感病毒进行了抗原性和基因特性的研究。用单克隆抗体对血凝素-酯酶糖蛋白的抗原性分析表明，分离物可分为三个不同的组，分别与C/Yamagata/26/81，C/Aichi/1/81和C/密西西比/80密切相关。总vRNA的T1-寡核苷酸指纹图谱显示，属于C/Yamagata/26/81病毒群的6个分离株的基因组彼此非常相似，但与1988/1990年分离株（C/Yamagata/10/89除外）的基因组有很大差异。比较7种RNA的全部或部分核苷酸序列。三株片段（C/宫城/3/91、C/宫城/9/91和C/宫城/2/92）代表C/Yamagata/26/81病毒组的1991/1993毒株与1980/1989年期间从人和猪获得的先前C型流感分离株的比较显示，1991/1993毒株，如C/Yamagata/10/89，与1981/1982年在中国北京从猪中分离的病毒比与任何从人中分离的病毒更密切相关。这一观察结果强烈表明，人类和猪之间的丙型流感病毒种间传播在自然界中已经发生，尽管目前还不知道该病毒是从猪传播给人类还是从人传播给猪。(C)1997年Elsevier Science B.V.
The antigenic and genetic characteristics of the 18 human strains of influenza C virus isolated in Yamagata and Sendai Cities, Japan between January 1991 and February 1993 were investigated. Antigenic analysis with monoclonal antibodies to the hemagglutinin-esterase glycoprotein showed that the isolates could be divided into three distinct groups closely related to C/Yamagata/26/8l, C/Aichi/1/81 and C/Mississippi/80, respectively. T1-oligonucleotide fingerprinting of total vRNA revealed that the six isolates belonging to the C/Yamagata/26/81 virus group had the genomes greatly similar to one another but considerably different from those of the 1988/1990 isolates (except C/Yamagata/10/89) of the same antigenic group. Comparison of total or partial nucleotide sequences of the seven RNA. segments of the three strains (C/Miyagi/3/91, C/Miyagi/9/91 and C/Miyagi/2/92) representative of the 1991/1993 strains of the C/Yamagata/26/81 virus group with those of the previous influenza C isolates obtained from humans and pigs during 1980/1989 showed that the 1991/1993 strains, like C/Yamagata/10/89, are more closely related to viruses isolated from pigs in Beijing, China in 1981/1982 than to any of the isolates from humans. This observation suggests strongly that interspecies transmission of influenza C virus between humans and pigs has occurred in nature, although it is not known whether the virus has been transmitted from pigs to humans or from humans to pigs. (C) 1997 Elsevier Science B.V.