Streptokinase as a Mediator of Acute Post-Streptococcal Glomerulonephritis in an Experimental Mouse Model
Streptokinase as a Mediator of Acute Post-Streptococcal Glomerulonephritis in an Experimental Mouse Model
复制标题
链激酶作为实验小鼠模型中急性链球菌后肾小球肾炎的介质
DOI：
10.1128/iai.66.1.315-321.1998
复制
发表时间：
1998
影响因子：
3.1
通讯作者：
S. Holm
中科院分区：
文献类型：
--
作者：
A. Nordstrand;M. Norgren;J. Ferretti;S. Holm
A组链球菌感染后有时会出现急性链球菌感染后肾小球肾炎（APSGN）。为了测试链激酶在这种疾病的发病机制中的重要性，肾炎分离株NZ131的同基因菌株，不同之处仅在于产生肾炎相关ska1基因型的链激酶的能力，用于感染APSGN的小鼠组织笼模型。链激酶的产生被认为是菌株诱导小鼠APSGN能力的先决条件。此外，在感染致肾炎NZ131和EF514菌株的小鼠肾脏中证实了链激酶。感染非致肾炎菌株S84后，既没有观察到链激酶也没有观察到C3沉积。感染后4天即可检测到肾小球中链激酶的沉积。这些发现为链激酶通过肾小球沉积启动肾炎过程的假设提供了支持，肾小球沉积导致补体级联的局部激活。肾组织中链激酶的检测随着肾小球细胞增生程度的增加而增加。因此，病理过程的严重程度可能是链激酶沉积程度的反映。
ABSTRACT Group A streptococcal infections are sometimes followed by the inflammatory kidney disease acute poststreptococcal glomerulonephritis (APSGN). To test the importance of streptokinase in the pathogenesis of this disease, isogenic strains of the nephritis isolate NZ131, differing only in the ability to produce streptokinase of the nephritis-associated ska1 genotype, were used for infection in a mouse tissue cage model for APSGN. Streptokinase production was found to be a prerequisite for the capacity of the strain to induce APSGN in mice. In addition, streptokinase was demonstrated in the kidneys of mice infected with the nephritogenic NZ131 and EF514 strains. After infection with the nonnephritogenic strain S84, neither streptokinase nor C3 deposition were observed. Deposition of streptokinase in the glomeruli was detected as soon as 4 days after infection. These findings provide support for the hypothesis that streptokinase initiates the nephritis process by glomerular deposition, which leads to local activation of the complement cascade. Detection of streptokinase in kidney tissue increased with the degree of glomerular hypercellularity. Thus, the severity of the pathological process may be a reflection of the degree of streptokinase deposition.
用质粒和线性 DNA 电转化化脓性链球菌。
影响因子：
2.1
作者：
Simon,D;Ferretti,JJ
通讯作者：
Ferretti,JJ
未能在急性链球菌感染后肾小球肾炎患者的血清或肾活检标本中检测到链球菌链激酶的独特反应性。
影响因子：
1.1
作者：
Mezzano,S;Burgos,E;Mahabir,R;Kemeny,E;Zabriskie,JB
通讯作者：
Zabriskie,JB