Nitric oxide levels in wound fluid may reflect the healing trajectory
Nitric oxide levels in wound fluid may reflect the healing trajectory
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DOI：
10.1111/wrr.12048
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发表时间：
2013-05-01
影响因子：
2.9
通讯作者：
Boykin, Joseph V., Jr.
中科院分区：
文献类型：
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作者：
Bernatchez, Stephanie F.;Menon, Vinod;Boykin, Joseph V., Jr.
我们分析了一氧化氮代谢物（硝酸盐和亚硝酸盐，NOx）和其他生物标志物在人类伤口液和相关的这些标志物与伤口愈合状态（进展或恶化）的基础上，患者的伤口历史。在清洁前和清洁后从各种病因的伤口患者中收集样品，并分析NOx、基质金属蛋白酶活性和弹性蛋白酶活性。开发了一种分析NOx的实验室方法，该方法可以在小至10 L的样品中检测至少5M。确定了一种不含硝酸盐的样品收集装置，以匹配这种新检测方法的灵敏度（当在如此小的体积中提取时，大多数测试的不含硝酸盐的产品所含的硝酸盐水平高于该检测限）。分析了清洁前和清洁后生物标志物值之间的相关性，以及生物标志物对伤口进展的诊断潜力。50个伤口提供了适合于NOx分析的样品。前和净化后的NOx值表现出良好的相关性（r=0.72），相关性不是很强的基质金属蛋白酶和弹性蛋白酶。数据分析表明，NOx代表区分恶化和进展性伤口的最佳代谢物，并表明使用NOx的两个临界点诊断测试优于单临界点测试，以鉴定从恶化伤口进展。
We analyzed nitric oxide metabolites (nitrate and nitrite, NOx) and other biomarkers in human wound fluids and correlated these markers with wound healing status (progressing or worsening) based on patient's wound history. Samples were collected pre- and postcleansing from patients with wounds of various etiologies and analyzed for NOx, matrix metalloproteinase activity, and elastase activity. A laboratory method was developed to analyze NOx which can detect at least 5M in samples as small as 10L. A nitrate-free sample collection device was identified to match the sensitivity of this new assay (most nitrate-free products tested contained nitrate levels higher than this detection limit when extracted in such a small volume). The correlation between pre- and postcleansing biomarker values, and the diagnostic potential of the biomarkers to wound progress were analyzed. Fifty wounds provided samples that were suitable for NOx analysis. The pre- and postcleansing values for NOx showed good correlation (r=0.72); the correlation was not very strong for matrix metalloproteinase and elastase. Data analysis showed that NOx represents the best metabolite to discriminate between worsening and progressing wounds, and suggested that a two cut point diagnostic test using NOx is better than a single cut point test to identify progressing from worsening wounds.