Basic fibroblast growth factor stimulates articular cartilage enlargement in young rats in vivo
Basic fibroblast growth factor stimulates articular cartilage enlargement in young rats in vivo
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DOI：
10.1002/jor.1100140215
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发表时间：
1996-03
影响因子：
2.8
通讯作者：
J. Shida;S. Jingushi;T. Izumi;A. Iwaki;Y. Sugioka
中科院分区：
文献类型：
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作者：
J. Shida;S. Jingushi;T. Izumi;A. Iwaki;Y. Sugioka
碱性成纤维细胞生长因子是一种有效的软骨细胞有丝分裂原，并影响其细胞外基质的蛋白质合成。为了研究其对体内正常发育的关节软骨的影响，我们将碱性成纤维细胞生长因子一次性注射到4周龄大鼠的膝关节中。将磷酸盐缓冲盐水类似地注射到对侧膝关节中作为对照。组织学分析表明，注射碱性成纤维细胞生长因子诱导关节软骨面积扩大，特别是在注射后第7天的髁突嵴区域。增大的程度具有剂量依赖性。应用增殖细胞核抗原检测法，对关节软骨中增殖细胞的定位和数量进行免疫组化分析。注射后第1天，与对照组相比，注射组关节中增殖细胞核抗原阳性细胞数显著增加，北方印迹分析显示，这些关节中α（II）前胶原信使RNA水平低于对照组。在第7天，注射过的关节中的信息增加，并且大于对照组。这表明，在发育中的关节软骨中增殖的软骨细胞对碱性成纤维细胞生长因子作出反应，导致软骨细胞增殖，随后软骨增大。
Basic fibroblast growth factor is a potent mitogen for chondrocytes and influences the protein synthesis of their extracellular matrix in vitro. To investigate its effect on normal developing articular cartilage in vivo, we injected basic fibroblast growth factor once into the knee joints of 4‐week‐old rats. Phosphate buffered saline was similarly injected into the contralateral knee joints as controls. A histological analysis showed that an injection of basic fibroblast growth factor induced enlargement of the articular cartilage area, especially in the condylar ridge region on day 7 after the injection. The extent of the enlargement was dose‐dependent. The localization and amount of proliferating cells in the articular cartilage were analyzed immunohistochemically by the detection of proliferating cell nuclear antigen. On day 1 after the injection, the number of cells positive for proliferating cell nuclear antigen increased significantly in the joints that were injected compared with the controls, and Northern blot analysis showed that the level of messenger RNA for α(II) procollagen was lower in these joints than in the controls. The message in the joints that had been injected increased on day 7, and it was greater than that in the controls. This suggests that proliferating chondrocytes in developing articular cartilage respond to basic fibroblast growth factor with a resulting proliferation of chondrocytes followed by enlargement of cartilage.