A comparative study of cell death using electrical capacitance measurements and dielectrophoresis
A comparative study of cell death using electrical capacitance measurements and dielectrophoresis
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DOI：
10.1016/j.enzmictec.2008.09.006
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发表时间：
2008-12-10
影响因子：
3.4
通讯作者：
Markx, Gerard H.
中科院分区：
文献类型：
--
作者：
Patel, Pareshkumar M.;Bhat, Aditya;Markx, Gerard H.
Z使用介电光谱和介电电泳测量细胞暴露于各种致命环境压力期间发生的介电特性的变化。结果表明，垂死和死亡酵母细胞的介电特性强烈依赖于诱导细胞死亡的方法。直接影响膜渗透性，进而影响膜电导率和内电导率的方法，导致悬浮电容和介电泳行为发生较大变化，而影响细胞内部但对细胞膜影响很小的方法，导致细胞介电特性很少或没有变化。研究结果表明，根据诱导细胞死亡的方法，介电光谱可能并不总是能够观察到活细胞和非活细胞之间的差异，并且介电电泳并不总是能够将活细胞与非活细胞分开。 (C) 2008 Elsevier Inc. 保留所有权利。
ZThe changes in the dielectric properties of cells that occur during their exposure to various lethal environmental stresses were measured using both dielectric spectroscopy and dielectrophoresis. It is shown that the dielectric properties of both dying and dead yeast cells were strongly dependent on the method used to induce cell death. Methods which directly affected the membrane permeability, and consequently the membrane conductivity and internal conductivity, resulted in large changes in the suspension capacitance and dielectrophoretic behaviour, whilst methods which affected the cell interior but had little effect on the cell membrane resulted in few or no changes in the dielectric properties of the cells. The findings indicate that, depending on the method by which cell death is induced, dielectric spectroscopy may not always be able to observe differences between viable and non-viable cells, and that dielectrophoresis will not always be able to separate viable from non-viable cells. (C) 2008 Elsevier Inc. All rights reserved.