POTENTIAL PROBLEMS WITH THE RELIABILITY OF PCR BASED DIAGNOSTIC METHODS RELATED TO PLANT VIRUSES SEQUENCE VARIATION
POTENTIAL PROBLEMS WITH THE RELIABILITY OF PCR BASED DIAGNOSTIC METHODS RELATED TO PLANT VIRUSES SEQUENCE VARIATION
复制标题
与植物病毒序列变异相关的基于 PCR 的诊断方法的可靠性的潜在问题
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
T. Malinowski
中科院分区：
文献类型：
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作者：
T. Malinowski
选定的问题有关的非均匀性的植物病毒序列变异体存在于受感染的植物和目前的诊断方法的不确定性进行了说明和讨论。证明了一些引物不能与苹果褪绿叶斑病毒（ACLSV）和李痘病毒（PPV）的非典型分离物/株（有效）反应。另一方面，对归类为D型的PPV分离株L5的有问题的阳性反应，但显示出一些非典型反应，进行了简要的介绍和分析。最后，对植物病毒诊断领域中如何解决这些问题提出了一些建议。
Selected problems related to non-uniformity of plant virus sequence variants present in infected plants and imperfectness of current diagnostic methods are illustrated and discussed. A failure of some primers to react (efficiently) with atypical isolates/strains of Apple chlorotic leaf spot virus (ACLSV) and Plum pox virus (PPV) is demonstrated. On the other hand, problematic positive reactions of PPV isolate L5 classified to D-type, but showing some atypical reaction, are presented and analysed briefly. Some recommendations how to address these problems in the field of plant viruses diagnostics are given.