Complete nucleotide sequence of the 6 kb element and conserved cytochrome b gene sequences among Indian isolates of Plasmodium falciparum
Complete nucleotide sequence of the 6 kb element and conserved cytochrome b gene sequences among Indian isolates of Plasmodium falciparum
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DOI：
10.1016/s0020-7519(01)00218-1
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发表时间：
2001-08-01
影响因子：
4
通讯作者：
Sharma, YD
中科院分区：
文献类型：
--
作者：
Sharma, I;Rawat, DS;Sharma, YD
疟疾寄生虫含有一个具有 14 条染色体的核基因组和两个大小为 6 kb 和 35 kb 的染色体外 DNA 分子。最小的基因组（称为 6 kb 元件或线粒体 DNA）已从几种恶性疟原虫分离株中进行了测序，因为这是潜在的药物靶点。在这里，我们描述了来自印度恶性疟原虫分离株的该元件的完整核苷酸序列。它的大小为 5967 bp，与其他分离株的 6 kb 元件显示出 99.6% 的同源性。该元件包含线粒体蛋白的三个开放阅读框——细胞色素氧化酶亚基 I (CoI)、亚基 III (CoIII) 和细胞色素 b (Cyb)，这些蛋白被发现在寄生虫的血液阶段表达。我们还对几个印度恶性疟原虫分离株的整个 cyb 基因进行了测序。该基因的突变率非常低，因为 14 个分离株中有 12 个显示出相同的序列。只有一个分离株显示出五个氨基酸的最大变化，而另一个分离株仅显示出一个氨基酸的变化。然而，印度分离株均未显示出与各种药物耐药性相关的 cyb 氨基酸发生任何变化，因为这些药物在印度尚未普遍使用。 (C) 2001 年澳大利亚寄生虫学协会。由 Elsevier Science Ltd 出版。保留所有权利。
The malaria parasite contains a nuclear genome with 14 chromosomes and two extrachromosomal DNA molecules of 6 kb and 35 kb in size. The smallest genome, known as the 6 kb element or mitochondrial DNA, has been sequenced from several Plasmodium falciparum isolates because this is a potential drug target. Here we describe the complete nucleotide sequence of this element from an Indian isolate of P. falciparum. It is 5967 bp in size and shows 99.6% homology with the 6 kb element of other isolates. The element contains three open reading frames for mitochondrial proteins-cytochrome oxidase subunit I (CoI), subunit III (CoIII) and cytochrome b (Cyb) which were found to be expressed during blood stages of the parasite. We have also sequenced the entire cyb gene from several Indian isolates of P. falciparum. The rate of mutation in this gene was very low since 12 of 14 isolates showed the identical sequence. Only one isolate showed a maximum change in five amino acids whereas the other isolate showed only one amino acid change. However, none of the Indian isolates showed any change in those amino acids of cyb which are associated with resistance to various drugs as these drugs are not yet commonly used in India. (C) 2001 Australian Society for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.