P53 QUANTITATIVE IMMUNOCYTOCHEMICAL ANALYSIS IN BREAST CARCINOMAS
P53 QUANTITATIVE IMMUNOCYTOCHEMICAL ANALYSIS IN BREAST CARCINOMAS
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DOI：
10.1016/0046-8177(95)90032-2
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发表时间：
1995-02-01
期刊：
影响因子：
3.3
通讯作者：
PIANA, L
中科院分区：
文献类型：
--
作者：
CHARPIN, C;DEVICTOR, B;PIANA, L
采用PAI、1801 p53单克隆抗体和链亲和素生物素过氧化物酶复合物对200例乳腺癌冷冻切片进行了研究。通过处理数字化显微图像评估免疫沉淀的密度分析。48%的肿瘤核内可见P53。一些肿瘤（91例中的14例）在石蜡切片上平行检测为阴性，但在冷冻切片上呈阳性。图像分析显示，石蜡切片上抗p53阳性表面及染色强度降低（P < 0.01）。p53肿瘤表达与患者年龄、肿瘤大小、腋窝淋巴结状态、HER-2/neu和组织蛋白酶D表达以及核形态计量参数无关。然而，p53与高组织学分级（P < 0.01）、雌激素受体（ER） （P = 0.0015）和孕激素（PR） （P = 0.0065）抗原位点缺乏、pS2检测（P = 0.03）、Ki-67高免疫反应性（P = 0.018）、大银染核桃核组织区（AgNOR）核表面比（P < 0.02）、高倍体程度（P < 0.03）相关，且多发见于痤疮癌。结果表明，p53在乳腺癌中的表达并不是一个完全独立的预后指标，如果程序标准化，特别是在使用冷冻切片和免疫检测的图像分析处理方面，可以可靠地评估p53在乳腺癌中的表达的临床相关性和预后意义。版权所有(C) 1995由W.B.桑德斯公司
A series of 200 breast carcinomas was investigated on frozen sections using PAI, 1801 p53 monoclonal antibody and streptavidin biotin peroxidase complex. Densitometric analysis of the immunoprecipitates was assessed by processing digitized microscopic images. p53 was observed in the nucleus of 48% of the tumors. Some tumors (14 of 91) tested in parallel on paraffin sections were negative, although positive on frozen sections. Image analysis showed that the surfaces positive with anti-p53 and the staining intensity were decreased (P < .01) on paraffin sections. The p53 tumor expression was independent of patient age, tumor size, axillary lymph node status, HER-2/neu and cathepsin D expression, and nuclear morphometric parameters. However, p53 correlated with high histological grade (P < .01), lack of estrogen receptor (ER) (P = .0015) and progesterone (PR) (P = .0065) antigenic sites, pS2 detection (P = .03), high Ki-67 immunoreactivity (P = .018), large silver-stained nucleolar organizer region (AgNOR) nuclear surface ratio (P < .02), and degree of hyperploidy (P < .03), and was more often observed in the comedocarcinomas, The results suggest that p53 expression in breast carcinomas is not a totally independent prognostic indicator and that the clinical relevance and prognostic significance of p53 expression in breast carcinomas can be reliably assessed provided that the procedures are standardized, particularly with regard to the use of frozen sections and image analysis processing of the immunodetection. Copyright (C) 1995 by W.B. Saunders Company