Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments
Inhibition of bacterial adhesion on PVC endotracheal tubes by RF-oxygen glow discharge, sodium hydroxide and silver nitrate treatments
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DOI：
10.1016/j.biomaterials.2003.08.053
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发表时间：
2004-05-01
期刊：
影响因子：
14
通讯作者：
Mathieu, HJ
中科院分区：
文献类型：
--
作者：
Balazs, DJ;Triandafillu, K;Mathieu, HJ
对医用级聚氯乙烯（PVC）进行化学改性，以研究单价银的掺入如何影响铜绿假单胞菌的粘附和定植。研究的改性包括射频-氧气（RF-O-2）辉光放电预功能化，然后在氢氧化钠和硝酸银溶液中进行两步湿处理。X射线光电子能谱（XPS）分析和接触角的测量被用来调查的化学性质和表面润湿性的膜以下的每个步骤的修改。XPS分析证明，天然PVC的RF-O-2等离子体预官能化可再现地增加了代表PVC添加剂的官能团的量，包括醚/醇、酯和羧基。更具体地，我们证明了在RF-0 -2等离子体预官能化步骤之后，代表天然PVC的邻苯二甲酸酯和羧酸锌添加剂的O-C-O基团增加了两倍。尽管RF-O-2预官能化对NaOH/AgNO 3处理的基材的银含量没有影响，但对于在生产批次中不具有可再现表面的生物材料产品，这种改性是必要的。XPS分析还表明，用氢氧化钠（NaOH）皂化酯，如PVC的邻苯二甲酸酯添加剂，是一种简单的不可逆水解方法，其产生羧酸钠和邻苯二甲酸钠盐。将天然PVC暴露于NaOH中，由于脱氯，导致表面亲水性增加（从约90度至约60度）。在硝酸银中进一步温育后的XPS分析表明，当在用单价含银溶液进行第二次处理后用银代替羧酸钠的钠时，可以捕获银离子。在天然PVC上产生银盐导致超疏水（> 120度）表面。使用NaOH和AgNO 3湿处理的化学改性完全抑制了铜绿假单胞菌的四种菌株对天然和氧预官能化PVC的细菌粘附，并且有效地防止了更长时间（72 h）的定殖。我们的研究结果表明，结合银离子的表面改性将非常有效地减少细菌定植到医疗器械。(C)2003 Elsevier Ltd.保留所有权利。
Medical-grade poly(vinyl chloride) (PVC) was chemically modified to study how the incorporation of monovalent silver influences Pseudomonas aeruginosa adhesion and colonization. The modification investigated consisted of a radio frequency-oxygen (RF-O-2) glow discharge pre-functionalization, followed by a two-step wet-treatment in sodium hydroxide and silver nitrate solutions. X-ray photoelectron spectroscopy (XPS) analysis and contact angle measurements were used to investigate the chemical nature and surface wettability of the films following each step of the modification. XPS analysis proved that the RF-O-2 plasma pre-functionalization of native PVC reproducibly increased the amount of functional groups representative of PVC additives, including ether/alcohol, esters and carboxyl groups. More specifically, we demonstrated that the O-C-O groups representative of the phthalic ester and zinc carboxylate additives identified for native PVC increased by two-fold following the RF-O-2 plasma pre-functionalization step. Although RF-O-2 pre-functionalization did not have an effect on the silver content of the NaOH/AgNO3 treated substrates, such a modification was necessary for biomaterial products that did not have reproducible surfaces amongst production lots. XPS analysis also demonstrated that saponification with sodium hydroxide (NaOH) of esters, like those of the phthalic ester additives of PVC is a simple, irreversible method of hydrolysis, which produced sodium carboxylate and sodium phthalate salts. Exposure of native PVC to NaOH resulted in an increased surface hydrophilicity (from ca 90degrees to ca 60degrees) due to dechlorination. XPS analysis following further incubation in silver nitrate demonstrated that silver ions can be trapped when the sodium of sodium carboxylate is replaced by silver after performing a second treatment with a monovalent silver-containing solution. The creation of silver salt on native PVC resulted in an ultra-hydrophobic (> 120degrees) surface. The chemical modifications using NaOH and AgNO3 wet treatments completely inhibited bacterial adhesion of four strains of P. aeruginosa to both native and oxygen-pre-functionalized PVC, and efficiently prevented colonization over longer periods (72 h). Our results suggest that surface modifications that incorporate silver ions would be extremely effective at reducing bacterial colonization to medical devices. (C) 2003 Elsevier Ltd. All rights reserved.