Click Reaction on Solid Phase Enables High Fidelity Synthesis of Nucleobase-Modified DNA
Click Reaction on Solid Phase Enables High Fidelity Synthesis of Nucleobase-Modified DNA
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DOI：
10.1021/acs.bioconjchem.5b00668
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发表时间：
2016-03-01
影响因子：
4.7
通讯作者：
Mayer, Guenter
中科院分区：
文献类型：
--
作者：
Tolle, Fabian;Rosenthal, Malte;Mayer, Guenter
通过点击化学（CuAAC）的核酸的合成后功能化已经看到了巨大的实现，扩展了核碱基修饰的核酸在荧光标记、纳米技术和体外选择等领域的适用性。然而，通过固相合成生产大量高密度官能化材料受到与碱性后处理条件相关的氧化副产物形成的阻碍。在本文中，我们描述了一种用于高保真大规模生产核碱基修饰的核酸的快速且具有成本效益的方案，以最近描述的核碱基修饰的适体为例。
The post-synthetic functionalization of nucleic acids via click chemistry (CuAAC) has seen tremendous implementation, extending the applicability of nucleobase-modified nucleic acids in fields like fluorescent labeling, nanotechnology, and in vitro selection. However, the production of large quantities of high-density functionalized material via solid phase synthesis has been hampered by oxidative by-product formation associated with the alkaline workup conditions. Herein, we describe a rapid and cost-effective protocol for the high fidelity large-scale production of nudeobase-modified nucleic acids, exemplified with a recently described nucleobase-modified aptamer.