A Label-Free Proteomic Analysis on Competent Larvae and Juveniles of the Pacific Oyster Crassostrea gigas.
A Label-Free Proteomic Analysis on Competent Larvae and Juveniles of the Pacific Oyster Crassostrea gigas.
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太平洋牡蛎感受态幼虫和幼体的无标记蛋白质组学分析
DOI：
10.1371/journal.pone.0135008
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Huan P;Wang H;Liu B
目前对双壳类变态的分子机制的认识还很零散，需要进行全面的描述。本研究采用大规模无标记蛋白质组学方法，对太平洋牡蛎（Crassostrea gigas）的适能幼体（CL）和幼体（JU）的蛋白质组进行了描述和比较。共鉴定了788种蛋白质：CL蛋白质组中392种，JU蛋白质组中636种。从每个样品的蛋白质组的基因本体论分析揭示了活跃的代谢过程中的两个阶段。进一步的定量分析揭示了117个蛋白质在两个样品之间差异表达。这些蛋白质被分为八类：细胞骨架和细胞粘附，蛋白质合成和降解，免疫和应激反应，特定组织的发育，信号调节，代谢和能量供应，运输和其他蛋白质。使用实时PCR检测的验证实验证实，30个检测基因中有20个在mRNA和蛋白质水平上表现出相同的趋势。这些差异表达的蛋白质可能在变态过程中和变态后的组织重塑、信号转导和器官发育中发挥作用。一些差异表达的蛋白质，如糜蛋白酶，提出了新的作用。本研究结果为中国对虾变态和变态后的发育提供了一个新的认识。在蛋白质水平上。对差异表达蛋白功能的进一步研究将有助于对双壳贝类变态的深入了解。
Current understandings on the molecular mechanisms underlying bivalve metamorphosis are still fragmentary, and a comprehensive description is required. In this study, using a large-scale label-free proteomic approach, we described and compared the proteomes of competent larvae (CL) and juveniles (JU) of the Pacific oyster, Crassostrea gigas. A total of 788 proteins were identified: 392 in the CL proteome and 636 in the JU proteome. Gene Ontology analysis of the proteome from each sample revealed active metabolic processes in both stages. Further quantitative analyses revealed 117 proteins that were differentially expressed between the two samples. These proteins were divided into eight groups: cytoskeleton and cell adhesion, protein synthesis and degradation, immunity and stress response, development of particular tissues, signal regulation, metabolism and energy supply, transport, and other proteins. A certification experiment using real-time PCR assay confirmed 20 of 30 examined genes exhibited the same trends at the mRNA and protein levels. The differentially expressed proteins may play roles in tissue remodeling, signal transduction, and organ development during and after metamorphosis. Novel roles were proposed for some differentially expressed proteins, such as chymotrypsin. The results of this work provide an overview of metamorphosis and post-metamorphosis development of C. gigas at the protein level. Future studies on the functions of the differentially expressed proteins will help to obtain a more in-depth understanding of bivalve metamorphosis.