Proteomic Analysis of Lipid Accumulation in Chlorella protothecoides Cells by Heterotrophic N Deprivation Coupling Cultivation
Proteomic Analysis of Lipid Accumulation in Chlorella protothecoides Cells by Heterotrophic N Deprivation Coupling Cultivation
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DOI：
10.1021/ef4000177
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发表时间：
2013-07
期刊：
影响因子：
5.3
通讯作者：
Yuqin Li;Zhengqiu Yuan;J. Mu;Di Chen;Bo Feng
中科院分区：
文献类型：
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作者：
Yuqin Li;Zhengqiu Yuan;J. Mu;Di Chen;Bo Feng
利用异养脱氮（HND）技术培养原壳小球藻（Chlorella protothecoides），获得了具有生物柴油潜力的微藻油脂。第一，C。异养培养原壳类以获得高生物量，然后将肉汤转移到N剥夺环境中进行脂质积累。本研究的目的是研究C.原壳细胞并鉴定负责HND脂质储存的分子途径。在240小时后，约72%的生物质（13.89 g/L）积累为脂质，这相当于10.0 g/L的脂质生产率。该结果表示与来自简单异养模式的脂质产量相比增加了79.5%。此外，在HND培养的藻细胞中成功鉴定了33个改变的蛋白质，其中13个蛋白质在光合作用、蛋白质合成与折叠、基因调控和脂肪酸β-氧化中表达下调; 15个蛋白质在HND培养的藻细胞中表达上调。
The heterotrophic nitrogen (N) deprivation (HND) was the first employed to culture Chlorella protothecoides for microalgal lipids, which are regarded as one of the most promising feedstocks for biodiesel production. First, C. protothecoides was cultivated heterotrophically to achieve high biomass, and the broth was then transferred to N deprivation environment for lipid accumulation. This study aims to investigate proteomic changes in C. protothecoides cells and identify the molecular pathways responsible for lipid storage with HND. Approximately 72% of biomass (13.89 g/L) accumulated as lipids after 240 h, which equates to a lipid productivity of 10.0 g/L. This result represents an increase of 79.5% compared with the lipid yield from the simple heterotrophic mode. Furthermore, 33 altered proteins in HND-cultured algal cells were successfully identified, including 13 down-regulated proteins involved in photosynthesis, protein synthesis and folding, gene regulation and β-oxidation of fatty acids; 15 up-reg...