The Staphylococcus aureus KdpDE Two-Component System Couples Extracellular K+ Sensing and Agr Signaling to Infection Programming
The Staphylococcus aureus KdpDE Two-Component System Couples Extracellular K+ Sensing and Agr Signaling to Infection Programming
复制标题
金黄色葡萄球菌 KdpDE 双组分系统将细胞外 K 感应和 Agr 信号传导与感染编程结合起来
DOI：
10.1128/iai.01180-10
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发表时间：
2011-06-01
影响因子：
3.1
通讯作者：
Sun, Baolin
中科院分区：
文献类型：
--
作者：
Xue, Ting;You, Yibo;Sun, Baolin
Kdp系统在细菌中广泛分布。在大肠杆菌中，Kdp - ATP酶是一种高亲和力的钾离子摄取系统，其表达受KdpDE双组分系统的激活，以应对钾离子限制或盐胁迫。然而，关于该系统在许多细菌中的作用信息仍然不清楚。在此我们证明金黄色葡萄球菌中的KdpFABC不是主要的钾离子转运体，并且KdpDE的主要功能与钾离子转运无关，而是通过感知外部钾离子浓度来调节一系列毒力因子的转录，这表明该细菌可能通过在不同环境中感知特定的外部钾离子刺激来调节其感染状态。我们的研究结果进一步揭示金黄色葡萄球菌的KdpDE受Agr/RNAIII系统上调，这表明KdpDE可能是一种重要的毒力调节因子，在该细菌的致病过程中协调外部钾离子感知和Agr信号传导。
ABSTRACT The Kdp system is widely distributed among bacteria. In Escherichia coli, the Kdp-ATPase is a high-affinity K+ uptake system and its expression is activated by the KdpDE two-component system in response to K+ limitation or salt stress. However, information about the role of this system in many bacteria still remains obscure. Here we demonstrate that KdpFABC in Staphylococcus aureus is not a major K+ transporter and that the main function of KdpDE is not associated with K+ transport but that instead it regulates transcription for a series of virulence factors through sensing external K+ concentrations, indicating that this bacterium might modulate its infectious status through sensing specific external K+ stimuli in different environments. Our results further reveal that S. aureus KdpDE is upregulated by the Agr/RNAIII system, which suggests that KdpDE may be an important virulence regulator coordinating the external K+ sensing and Agr signaling during pathogenesis in this bacterium.