ADHESION TO AND INVASION OF HEP-2 CELLS BY CAMPYLOBACTER SPP
ADHESION TO AND INVASION OF HEP-2 CELLS BY CAMPYLOBACTER SPP
复制标题
DOI：
10.1128/iai.57.10.2984-2990.1989
复制
发表时间：
1989-10-01
影响因子：
3.1
通讯作者：
JOENS, LA
中科院分区：
文献类型：
--
作者：
KONKEL, ME;JOENS, LA
在体外测试了21株分离株对HEp-2细胞的粘附和侵袭能力。在测试的21种生物中，2种没有侵入HEp-2细胞，其中1种没有粘附在上皮细胞上。空肠弯曲杆菌临床分离株比非临床分离株更具侵袭性。当用细胞松弛素B处理HEp-2细胞时，空肠梭菌的侵袭性降低，表明宿主细胞积极参与了这些生物的摄取。当弯曲杆菌全细胞裂解物被吸附在HEp-2细胞单层上时，细胞内空肠梭菌分离物的数量减少。实验还确定了弯曲杆菌入侵抗原表达的功能位点。后高碘酸钠氧化裂解物显著影响其抑制能力。这意味着弯曲杆菌侵入性配体似乎依赖于完整的碳水化合物部分。
Twenty-one isolates were tested for their ability to adhere to and invade HEp-2 cells in vitro. Of the 21 organisms tested, 2 did not invade the HEp-2 cells, and 1 of these did not adhere to the epithelial cells. Campylobacter jejuni clinical isolates were more invasive than the nonclinical strains that were tested. When HEp-2 cells were treated with cytochalasin B, the invasiveness of C. jejuni was reduced, indicating active participation of the host cell in the uptake of these organisms. The number of intracellular C. jejuni isolates decreased when Campylobacter whole-cell lysates were adsorbed onto HEp-2 cell monolayers. Experiments were also conducted to identify the functional sites of the antigens responsible for expression of Campylobacter invasion. Oxidation of lysates with sodium meta-periodate significantly affected its inhibitory capacity. This implies that the Campylobacter invasive ligand appears to be dependent upon an intact carbohydrate moiety.