Differential Storage of Prolactin, Granins (Chromogranin B and Secretogranin II), and Constitutive Secretory Markers in Rat Pituitary GHC Cells (*)
Differential Storage of Prolactin, Granins (Chromogranin B and Secretogranin II), and Constitutive Secretory Markers in Rat Pituitary GHC Cells (*)
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大鼠垂体 GHC 细胞中催乳素、颗粒蛋白（嗜铬蛋白 B 和分泌颗粒蛋白 II）和组成型分泌标记物的差异储存 (*)
DOI：
--
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发表时间：
1996
影响因子：
4.8
通讯作者：
S. Gorr
中科院分区：
文献类型：
--
作者：
S. Gorr
大鼠垂体细胞系GHC通过调节分泌途径分泌颗粒素(嗜铬粒素B和分泌颗粒素II)和催乳素。催乳素的细胞内储存优先由雌激素、胰岛素和表皮生长因子的激素处理诱导。这项研究的目的是确定激素处理对颗粒素和结构性分泌标志物储存的影响。在激素处理和未处理的细胞中均能有效储存颗粒素(4h内分泌总量的17%)。分泌型碱性磷酸酶(SEAP)是一种截短的膜蛋白，不能进入分泌颗粒，糖胺聚糖是构成分泌途径的标志，在这两种条件下都有70%-80%的分泌。相比之下，激素处理和未处理细胞的催乳素相对分泌量分别为31%和68%。佛波酯和氯化钾能刺激未经处理的细胞分泌催乳素2.3倍，激素处理的细胞分泌催乳素5.5倍。相反，SEAP的分泌在处理和未处理的细胞中都被刺激了1.5倍，这与结构性分泌途径的分泌一致。激素处理和未处理的细胞均可检测到颗粒素的刺激分泌。这些结果表明，颗粒素和催乳素的储存受到不同的调节，构成的分泌途径不受激素处理的影响。
The rat pituitary cell line GHC secretes granins (chromogranin B and secretogranin II) and prolactin by the regulated secretory pathway. The intracellular storage of prolactin is preferentially induced by hormone treatment with estradiol, insulin, and epidermal growth factor. The goal of this study was to determine the effect of hormone treatment on storage of granins and constitutive secretory markers. The granins were efficiently stored in both hormone-treated and -untreated cells (17% of total secreted in 4 h). Secreted alkaline phosphatase (SEAP), a truncated membrane protein that would not be expected to enter secretory granules, and glycosaminoglycan, a marker for the constitutive secretory pathway, exhibited 70-80% secretion under both conditions. In comparison, the relative prolactin secretion was 31 and 68% from hormone-treated and -untreated cells, respectively. Phorbol ester and KCl stimulated prolactin secretion 2.3-fold from untreated cells and 5.5-fold from hormone-treated cells. In contrast, SEAP secretion was stimulated 1.5-fold from both treated and untreated cells, consistent with secretion by the constitutive secretory pathway. Stimulated secretion of granins was detected from both hormone-treated and -untreated cells. These results suggest that granin and prolactin storage are differentially regulated and that the constitutive secretory pathway is not affected by hormone treatment.
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DOI：
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发表时间：
1988
期刊：
Bone and mineral
影响因子：
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作者：
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DOI：
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影响因子：
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DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
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作者：
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Cohn,DV
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DOI：
10.1177/38.1.2403576
发表时间：
1990
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
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