Synergistic inhibition of LDL oxidation by phytoestrogens and ascorbic acid
Synergistic inhibition of LDL oxidation by phytoestrogens and ascorbic acid
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DOI：
10.1016/s0891-5849(00)00322-1
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发表时间：
2000-07-01
影响因子：
7.4
通讯作者：
Ursini, F
中科院分区：
文献类型：
--
作者：
Hwang, J;Sevanian, A;Ursini, F
越来越多的证据表明，低密度脂蛋白（LDL）的氧化修饰是动脉粥样硬化形成的重要决定因素，绝经后，女性冠心病的发病率与男性一样普遍。雌激素已被证明可以抑制低密度脂蛋白被氧化的敏感性，最近人们考虑使用植物雌激素作为雌激素替代疗法。在这项研究中，根据低密度脂蛋白氧化敏感性测量了三种主要植物雌激素：染料木黄酮、大豆黄酮和雌马酚的抗氧化活性。染料木黄酮、大豆黄酮和雌马酚水平的增加可抑制低密度脂蛋白氧化，并且在抗坏血酸存在下，这种抑制作用进一步增强。这些化合物表现出的协同作用对于植物雌激素治疗具有临床重要性，因为植物雌激素作为有效抗氧化剂的功效在其浓度完全在食用豆制品的受试者血浆中发现的范围内时是明显的。然而，这种协同作用，加上植物雌激素与过氧自由基的低反应性，表明除自由基清除反应之外的抗氧化机制解释了植物雌激素的抗氧化作用。推测了涉及植物雌激素与apoB-100相互作用的抑制LDL氧化的结构基础。 (C) 2000 爱思唯尔科学公司。
Increasing evidence indicates that oxidative modification of low-density lipoprotein (LDL) is an important determinant in atherogenesis, and following menopause, the incidence of coronary heart disease is as prevalent in women as it is in men. Estrogen has been demonstrated to inhibit the susceptibility of LDL to be oxidized, and more recently the use of phytoestrogens has been considered for estrogen replacement therapy. In this study the antioxidant activity of the three major phytoestrogens: genistein, daidzein, and equol were measured in terms of LDL oxidative susceptibility. Increasing levels of genistein, daidzein, and equol inhibited LDL oxidation, and this inhibitory effect was further enhanced in the presence of ascorbic acid. The synergism exhibited by these compounds is of clinical importance to phytoestrogen therapy since the efficacy of phytoestrogens as effective antioxidants is evident at concentration well within the range found in the plasma of subjects consuming soy products. However, this synergism, combined with the low reactivity of the phytoestrogens with peroxyl radicals, suggests that an antioxidant mechanism other then free radical scavenging reactions account for the phytoestrogen antioxidant effect, A structural basis for inhibition of LDL oxidation involving interaction of the phytoestrogens with apoB-100 is postulated. (C) 2000 Elsevier Science Inc.