Sub-2 μm porous and nonporous particles for fast separation in reversed-phase high performance liquid chromatography
Sub-2 μm porous and nonporous particles for fast separation in reversed-phase high performance liquid chromatography
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DOI：
10.1016/j.chroma.2006.07.042
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发表时间：
2006-10-27
影响因子：
4.1
通讯作者：
Lee, Milton L.
中科院分区：
文献类型：
--
作者：
Wu, Naijun;Liu, Yansheng;Lee, Milton L.
在填充柱液相色谱 (LC) 中实现快速有效分离的一种方法是使用填充有小颗粒的短柱来减少流动相中的涡流扩散和传质阻力。在本研究中，使用硝基甲烷和蛋白质作为分析物，在反相 LC 中比较了填充 1.5 和 3.0 μm 无孔和多孔颗粒的色谱柱的效率。当使用硝基甲烷作为溶质时，无孔颗粒在高线速度下提供了更高的整体效率；然而，当颗粒尺寸从3μm减小到1.5μm时，效率差异显着减小。 1.5μm的效率。当使用蛋白质α-胰凝乳蛋白酶原A（MW 25,000）作为溶质时，无孔颗粒在高线速度下明显高于1.5μm多孔颗粒。此外，对于含有40%至75%乙腈的水性流动相组合物，在填充有ACQUITY C-18多孔颗粒的柱中，戊基苯的平均保留因子比Micra C18无孔颗粒高约16倍。还对实际条件下多孔和无孔颗粒的压降、样品负载能力和分离能力进行了评估和比较。 (c) 2006 Elsevier B.V. 保留所有权利。
One approach to achieve fast and efficient separations in packed column liquid chromatography (LC) is to reduce eddy diffusion and mass transfer resistance in the mobile phase using short columns packed with small particles. In this study, efficiencies of columns packed with 1.5 and 3.0 mu m nonporous and porous particles were compared in reversed-phase LC using nitromethane and a protein as analytes. Nonporous particles provided overall higher efficiency at high linear velocities when nitromethane was used as solute; however, the efficiency difference diminished significantly when the particle size was reduced from 3 to 1.5 mu m. Efficiencies for 1.5 mu m. nonporous particles were considerably higher than those for 1.5 mu m porous particles at high linear velocities when a protein, alpha-chymotrypsinogen A (MW 25,000), was used as solute. In addition, the average retention factor for amylbenzene in a column packed with ACQUITY C-18 porous particles was approximately 16 fold higher than for Micra C18 nonporous particles for aqueous mobile phase compositions containing from 40 to 75% acetonitrile. Pressure drop, sample loading capacity, and separation power were also evaluated and compared for porous and nonporous particles under practical conditions. (c) 2006 Elsevier B.V. All rights reserved.