Multifunctional calcium phosphate based coatings on titanium implants with integrated trace elements
Multifunctional calcium phosphate based coatings on titanium implants with integrated trace elements
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DOI：
10.1088/1748-605x/ab5d7b
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发表时间：
2019-11
影响因子：
4
通讯作者：
C. Wolf-Brandstetter;R. Beutner;R. Hess;S. Bierbaum;Katrin Wagner;D. Scharnweber;U. Gbureck;C. Moseke
中科院分区：
文献类型：
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作者：
C. Wolf-Brandstetter;R. Beutner;R. Hess;S. Bierbaum;Katrin Wagner;D. Scharnweber;U. Gbureck;C. Moseke
几十年来，为恢复骨功能而开发的钛种植体的主要焦点是改善骨整合。由于抗生素耐药性的增加可能会带来植入物相关感染的风险，因此现在人们需要额外的抗菌特性。为此，通过电化学辅助沉积将微量元素铜和锌整合到磷酸钙基涂层中。据报道，除了抗菌活性之外，锌还可以通过趋化性吸引骨祖细胞，从而增加成骨分化，而铜则可以刺激血管生成。沉积量高达 68.9 ± 0.1 μg cm−2 的铜和 56.6 ± 0.4 μg cm−2 的锌；两种离子的共沉积不影响锌的含量，但略微增加了涂层中铜的含量。在无血清模拟体液中沉积的铜和锌物质的释放可以忽略不计。在含蛋白质的溶液中，观察到铜的爆发释放量高达 10 μg ml−1，而锌的连续释放长达 14 天。锌的存在有利于人间充质基质细胞以浓度依赖性方式粘附和生长，但对于中等铜含量的涂层来说，细胞毒性作用已经可见。然而，共沉积锌可以在一定程度上减轻铜的不利影响。大肠杆菌抗菌测试表明，不含铜或锌的透钙磷石上的附着细菌减少了 60%，但如果涂层含有这两种离子，则 12 小时后几乎没有细菌附着。高锌含量和中等铜含量的涂层具有总体上最好的多功能性能。
For decades, the main focus of titanium implants developed to restore bone functionality was on improved osseointegration. Additional antimicrobial properties have now become desirable, due to the risk that rising antibiotic resistance poses for implant-associated infections. To this end, the trace elements of copper and zinc were integrated into calcium phosphate based coatings by electrochemically assisted deposition. In addition to their antimicrobial activity, zinc is reported to attract bone progenitor cells through chemotaxis and thus increase osteogenic differentiation, and copper to stimulate angiogenesis. Quantities of up to 68.9 ± 0.1 μg cm−2 of copper and 56.6 ± 0.4 μg cm−2 of zinc were deposited; co-deposition of both ions did not influence the amount of zinc but slightly increased the amount of copper in the coatings. The release of deposited copper and zinc species was negligible in serum-free simulated body fluid. In protein-containing solutions, a burst release of up to 10 μg ml−1 was observed for copper, while zinc was released continuously for up to 14 days. The presence of zinc was beneficial for adhesion and growth of human mesenchymal stromal cells in a concentration-dependent manner, but cytotoxic effects were already visible for coatings with an intermediate copper content. However, co-deposited zinc could somewhat alleviate the adverse effects of copper. Antimicrobial tests with E. coli revealed a decrease in adherent bacteria on brushite without copper or zinc of 60%, but if the coating contained both ions there was almost no bacterial adhesion after 12 h. Coatings with high zinc content and intermediate copper content had the overall best multifunctional properties.