A population genomics insight by 2b-RAD reveals populations' uniqueness along the Italian coastline in Leptopsammia pruvoti (Scleractinia, Dendrophylliidae)
A population genomics insight by 2b-RAD reveals populations' uniqueness along the Italian coastline in Leptopsammia pruvoti (Scleractinia, Dendrophylliidae)
复制标题
DOI：
10.1111/ddi.12918
复制
发表时间：
2019-07-01
影响因子：
4.6
通讯作者：
Congiu, Leonardo
中科院分区：
文献类型：
--
作者：
Boscari, Elisa;Abbiati, Marco;Congiu, Leonardo
目的海洋生物建群（如珊瑚建造）是生物多样性研究的热点，通过为海洋生物提供碳调节、保护和保育场所，在生物多样性保护中发挥着重要的生态作用。因此，《欧洲联盟生境指令》将它们列为优先保护的生境。虽然它们的生态作用已经确立，但连通性模式仍然没有得到很好的研究，这是保护规划的一个限制。本研究开拓了一种新的方法，利用下一代测序技术分析海洋生物构造物种的连通性，这些物种通常缺乏合适的遗传标记。我们评估的基因组变异的日落杯珊瑚Leptopsammia pruvoti Lacaze-Duthiers，1897年，一个ahermatypic的，非zooxanthellate和孤独的石珊瑚种常见的珊瑚栖息地和分布在地中海的地理格局。意大利海岸线（地中海西部和中部）。方法我们应用限制性酶切位点相关2b-RAD方法对10个人群样本中超过1,000个高质量和筛选的单核苷酸多态性进行基因分型。结果结果表明，所有群体样本（包括相距约5 km的样本）的F-ST值均达到极显著水平，且存在强支持的遗传结构。此外，基因组数据表明，基因流动的最大障碍是在意大利半岛的西部（利古里亚-第勒尼安海）和东部（亚得里亚海）之间。主要结论L. pruvoti与在该地区调查的其他海洋生物建造者物种中发现的相似，但与在同一地理范围内许多鱼类和无脊椎动物中发现的微小差异形成强烈对比。总而言之，我们的研究结果强调了评估属于珊瑚栖息地的物种的连通性的重要性，由于其有限的扩散能力，它们可能需要特定的空间保护措施。
Aim Marine bioconstructions such as coralligenous formations are hotspot of biodiversity and play a relevant ecological role in the preservation of biodiversity by providing carbon regulation, protection and nursery areas for several marine species. For this reason, the European Union Habitat Directive included them among priority habitats to be preserved. Although their ecological role is well established, connectivity patterns are still poorly investigated, representing a limit in conservation planning. The present study pioneers a novel approach for the analysis of connectivity in marine bioconstructor species, which often lack suitable genetic markers, by taking advantage of next-generation sequencing techniques. We assess the geographical patterns of genomic variation of the sunset cup coral Leptopsammia pruvoti Lacaze-Duthiers, 1897, an ahermatypic, non-zooxanthellate and solitary scleractinian coral species common in coralligenous habitats and distributed across the Mediterranean Sea. Location The Italian coastline (Western and Central Mediterranean). Methods We applied the restriction site-associated 2b-RAD approach to genotype over 1,000 high-quality and filtered single nucleotide polymorphisms in 10 population samples. Results The results revealed the existence of a strongly supported genetic structure, with highly significant pairwise F-ST values between all the population samples, including those collected about 5 km apart from each other. Moreover, genomic data indicate that the strongest barriers to gene flow are between the western (Ligurian-Tyrrhenian Sea) and the eastern side (Adriatic Sea) of the Italian peninsula. Main conclusions The strong differentiation found in L. pruvoti is similar to that found in other species of marine bioconstructors investigated in this area, but it strongly contrasts with the small differences found in many fish and invertebrates at the same geographical scale. All in one, our results highlight the importance of assessing connectivity in species belonging to coralligenous habitats as, due to their limited dispersal ability, they might require specific spatial conservation measures.