Expression of tissue inhibitors of metalloproteinases (TIMPs) in gastric cancer
Expression of tissue inhibitors of metalloproteinases (TIMPs) in gastric cancer
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DOI：
10.1023/a:1005421713137
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发表时间：
2000-01-01
影响因子：
3.1
通讯作者：
Kim, SJ
中科院分区：
文献类型：
--
作者：
Joo, YE;Seo, KS;Kim, SJ
基质金属蛋白酶（Matrix metalloproteinases，MMPs）是参与肿瘤侵袭和转移的主要蛋白水解酶之一，被天然存在的金属蛋白酶组织抑制剂（tissue inhibitor of metalloproteinases，TIMPs）抑制。本研究选择了1992年在全南国立大学医院接受胃癌手术的65例患者。主要的选择标准是福尔马林固定和石蜡包埋的组织块的可用性和足够的临床随访，用于肿瘤特异性生存分析。本研究应用原位杂交和免疫组化方法检测TIMP-1和TIMP-2在胃癌组织中的表达，并分析其与临床病理参数的关系。TIMP-1和TIMP-2主要表达于瘤周间质细胞而非肿瘤细胞本身。免疫组化染色结果与原位杂交结果一致。TIMP-1免疫组化染色强度与肿瘤分期（P = 0.009）和患者生存期（P = 0.025）相关。然而，TIMP-2免疫组化间质染色强度与肿瘤分期（P = 0.339）和患者生存期（P 0.474）无关。TIMP-1表达的增加与胃癌分期的相关性反映了TIMP-1在预测胃癌侵袭行为中的作用。TIMP-2表达与临床病理参数无关。但TIMP-1的表达及TIMP-2在判断胃癌预后中可能的附加价值有待进一步探讨。
Matrix metalloproteinases (MMPs) are one of the major classes of proteolytic enzymes involved in tumor invasion and metastasis, being inhibited by naturally occurring tissue inhibitors of metalloproteinases (TIMPs). Sixty-five patients who underwent surgery for gastric cancer in 1992 at Chonnam National University Hospital were selected for this study. The primary selection criteria were the availability of formalin-fixed and paraffin-embedded blocks and sufficient clinical follow-up for tumor-specific survival analysis. In this study, we examined the expression of TIMP-1 and TIMP-2 in human gastric cancer tissue by in situ hybridization and immunohistochemistry, and the correlation between their expression and clinicopathological parameters. TIMP-1 and TIMP-2 expressions were detected predominantly in the peritumor stromal cells rather than tumor cells themselves. Immunohistochemical stainings were concordant with the result obtained by in situ hybridization. The intensity of TIMP-1 immunohistochemical stromal staining correlated with tumor stage (P = 0.009) and patient survival (P = 0.025). However, the intensity of TIMP-2 immunohistochemical stromal staining did not correlate with tumor stage (P = 0.339) and patient survival (P 0.474). The correlation between the increased TIMP-1 expression and cancer stage noted in this study reflects a role of TIMP-1 in predicting the aggressive behavior of gastric cancer. TIMP-2 expression did not correlate with clinicopathological parameters. However, expression of TIMP-1 and the possible additional value of TIMP-2 should be further explored in determining the prognosis of gastric cancer.