CHARACTERIZATION OF CDNAS FOR STYLAR TRANSMITTING TISSUE-SPECIFIC PROLINE-RICH PROTEINS IN TOBACCO
CHARACTERIZATION OF CDNAS FOR STYLAR TRANSMITTING TISSUE-SPECIFIC PROLINE-RICH PROTEINS IN TOBACCO
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DOI：
10.1111/j.1365-313x.1993.tb00018.x
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发表时间：
1993-01-01
期刊：
影响因子：
7.2
通讯作者：
WU, HM
中科院分区：
文献类型：
--
作者：
CHEUNG, AY;MAY, B;WU, HM
花的雌蕊是一个包含雌性配子体的特殊器官，为授粉和受精提供必要的结构。花粉沉积在亲和性植物的柱头表面，形成花粉管，花粉管穿过柱头乳头，在细胞间通过花柱向子房的胚珠生长。花粉管的生长主要局限于花柱中的传递组织。因此花柱传递组织对花粉细胞向子房的迁移起着极其重要的作用。我们分离了两个相关的cdna，分别是TTS- 1和TTS-2，它们来源于两个富含脯氨酸蛋白（PRP）编码的mrna，它们在烟草的传递组织中特异性积累。推导出的PRP序列与多种植物中发现的富含脯氨酸的细胞壁糖蛋白有相似之处。TTS-1和TTS-2 mrna在非常年轻的花蕾中被诱导，在花柱伸长最快的花发育后期积累最丰富，并在花期保持相对较高的水平。这些mrna在成熟的绿色水果中无法检测到。原位杂交表明，TTS-1和TTS-2 mRNA的积累仅限于花柱的传递组织。讨论了这些传递组织特异性PRPs在维持花柱结构完整性或该器官功能方面可能发挥的作用。
The pistil of flowers is a specialized organ which contains the female gametophytes and provides the structures necessary for pollination and fertilization. Pollen deposited on the stigmatic surface of a compatible plant germinates a pollen tube which penetrates the stigmatic papillae and grows intercellularly through the style towards the ovules in the ovary. Pollen tube growth is largely restricted to the transmitting tissue in the style. Therefore the stylar transmitting tissue is extremely important for the migration of the pollen cell towards the ovary. We have isolated two related cDNAs, transmitting tissue-specific (TTS)-l and TTS-2, derived from two proline-rich protein (PRP)-encoding mRNAs that accumulate specifically in the transmitting tissue of tobacco. The deduced PRP sequences share similarities with proline-rich cell wall glycoproteins found in a variety of plants. TTS-1 and TTS-2 mRNAs are induced in very young floral buds, accumulate most abundantly during the later stages of flower development when style elongation is the most rapid, and remain at relatively high levels at anthesis. These mRNAs become undetectable in maturing green fruits. In situ hybridization shows that TTS-1 and TTS-2 mRNA accumulation is restricted to the transmitting tissue of the style. The possible roles that these transmitting tissue-specific PRPs may play in maintaining the structural integrity of the style or in the function of this organ is discussed.