Aseptic cure of pulmonary paracoccidioidomycosis can be achieved after a previous subcutaneous immunization of susceptible but not resistant mice
Aseptic cure of pulmonary paracoccidioidomycosis can be achieved after a previous subcutaneous immunization of susceptible but not resistant mice
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DOI：
10.1016/j.micinf.2007.02.014
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发表时间：
2007-05-01
影响因子：
5.8
通讯作者：
Calich, Vera L. G.
中科院分区：
文献类型：
--
作者：
Arruda, Celina;Vaz, Celideia A. C.;Calich, Vera L. G.
副球孢子菌病是南美洲最重要的地方性真菌病，其小鼠模型模拟人类疾病：抗性与保留的细胞免疫相关，而T细胞无反应性与易感性相关。在本研究中，我们问是否以前的s.c.诱导强细胞免疫的感染将保护小鼠免受致命的肺部攻击。结果发现，易感小鼠而非抗性小鼠产生了免疫保护和感染的无菌治愈。免疫保护导致DTH无反应性逆转，抗体和肺IL- 12、IL-2和IL-4水平升高，表明平衡的1型/2型应答。相反，A/Sn感染和免疫没有观察到显著差异。耗竭实验表明，免疫保护需要CD 4（+）和CD 8（+）T细胞与IFN-γ和IL-12的协同作用。总之，这些观察结果表明，易感宿主可以发展杀菌免疫，并确定了主要的免疫要求，以控制继发性副球孢子菌病。(C)2007年Elsevier Masson SAS。All rights reserved.
The murine model of paracoccidioidomycosis, the most important South American endemic mycosis, mimics the human disease: resistance is associated with preserved cellular immunity while T-cell anergy is related with susceptibility. In the present study we asked whether a previous s.c. infection which induces strong cellular immunity would protect mice against a lethal pulmonary challenge. It was found that susceptible but not resistant mice developed immunoprotection and aseptic cure of infection. Immunoprotection led to reversal of DTH anergy, increased levels of antibodies and pulmonary IL- 12, IL-2 and IL-4 indicating a balanced type 1/type 2 response. On the contrary, no marked differences in A/Sn infection and immunity were observed. Depletion experiments showed that immunoprotection required the cooperative action of CD4(+) and CD8(+) T cells in association with IFN-gamma and IL-12. Altogether, these observations demonstrated that susceptible hosts can develop sterilizing immunity and defined the main immunological requirements to control secondary paracoccidioidomycosis. (C) 2007 Elsevier Masson SAS. All rights reserved.