β2 microglobulin-deficient (B2mnull) NOD/SCID mice are excellent recipients for studying human stem cell function
β2 microglobulin-deficient (B2mnull) NOD/SCID mice are excellent recipients for studying human stem cell function
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DOI：
10.1182/blood.v95.10.3102.010k14_3102_3105
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发表时间：
2000-05-15
期刊：
影响因子：
20.3
通讯作者：
Lapidot, T
中科院分区：
文献类型：
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作者：
Kollet, O;Peled, A;Lapidot, T
人类SCID再生细胞（SRC）的定义是基于它们在骨髓和淋巴细胞群中重新填充NODI SCID小鼠骨髓的功能能力。SRC在脐带血细胞中的频率为1 / 9.3 x 10(5)个单核细胞。我们报道，只有8 × 10(4)个人脐带血单个核细胞移植到NOD/SCID/B2m（null）小鼠中，导致小鼠骨髓多谱系分化，表明SRC频率比NOD/SCID小鼠高11倍以上。此外，从初次移植的NOD/SCID/B2m（null）小鼠骨髓中恢复的CD34（+）细胞仅2至5 × 10(3)个就足以移植继发性NOD/SCID/B2m（null）小鼠的SRC，这表明SRC可以自我更新。因此，通过NOD/SCID/B2m（null）小鼠作为受体，我们建立了一种能够移植原发性和继发性免疫缺陷小鼠骨髓的人类干细胞功能试验，提供了一种更类似于自体干细胞移植的模型。(C) 2000年由美国血液学会出版。
Human SCID repopulating cells (SRC) are defined based on their functional ability to repopulate the bone marrow of NODI SCID mice with both myeloid and lymphoid cell populations. The frequency of SRC in umbilical cord blood cells is 1 in 9.3 x 10(5) mononuclear cells. We report that as few as 8 x 10(4) human cord blood mononuclear cells transplanted into NOD/SCID/B2m(null) mice resulted in mutlilineage differentiation in the murine bone marrow, revealing a more than 11-fold higher SRC frequency than in NOD/SCID mice. Moreover, as few as 2 to 5 x 10(3) CD34(+) cells recovered from the bone marrow of primary transplanted NODI SCID mice were sufficient for engrafting secondary NOD/SCID/B2m(null) mice with SRC, suggesting SRC self-renewal. Thus, by using NOD/SCID/B2m(null) mice as recipients, we established a functional assay for human stem cells capable of engrafting the bone marrow of primary and secondary transplanted immune-deficient mice with SRC, providing a model that better resembles autologous stem cell transplantation. (C) 2000 by The American Society of Hematology.