Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions
Global dynamics of Escherichia coli phosphoproteome in central carbon metabolism under changing culture conditions
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DOI：
10.1016/j.jprot.2015.05.021
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发表时间：
2015-08-03
影响因子：
3.3
通讯作者：
Nielsen, Lars K.
中科院分区：
文献类型：
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作者：
Lim, SooA;Marcellin, Esteban;Nielsen, Lars K.
关于全球磷酸化事件在原核生物代谢控制中的作用知之甚少。通过对大肠杆菌中所有蛋白磷酸化事件进行详细分析，阐明了三种不同培养条件下蛋白磷酸化的动态变化。使用预定的反应监测，对对应于71种蛋白质的82种肽的磷酸化比率进行定量，以确定丝氨酸（S）、苏氨酸（T）和酪氨酸（Y）磷酸化事件是否在变化的培养条件下显示动态曲线。发现来自中心碳代谢的23种酶的磷酸化比率是动态的。所提供的数据有助于我们理解磷酸化在细菌代谢中的全球作用，并强调磷酸化是细菌代谢控制的重要但知之甚少的调节机制。(C)2015 Elsevier B.V.版权所有。
Little is known about the role of global phosphorylation events in the control of prokaryote metabolism. By performing a detailed analysis of all protein phosphorylation events previously reported in Escherichia coli, dynamic changes in protein phosphorylation were elucidated under three different culture conditions. Using scheduled reaction monitoring, the phosphorylation ratios of 82 peptides corresponding to 71 proteins were quantified to establish whether serine (S), threonine (T) and tyrosine (Y) phosphorylation events displayed a dynamic profile under changing culture conditions. The ratio of phosphorylation for 23 enzymes from central carbon metabolism was found to be dynamic. The data presented contributes to our understanding of the global role of phosphorylation in bacterial metabolism and highlight that phosphorylation is an important, yet poorly understood, regulatory mechanism of metabolism control in bacteria. (C) 2015 Elsevier B.V. All rights reserved.