Transcoronary gradients of HDL-associated MicroRNAs in unstable coronary artery disease
Transcoronary gradients of HDL-associated MicroRNAs in unstable coronary artery disease
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DOI：
10.1016/j.ijcard.2017.09.190
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发表时间：
2018-02-15
影响因子：
3.5
通讯作者：
Tabet, Fatiha
中科院分区：
文献类型：
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作者：
Choteau, Sebastien A.;Torres, Luisa F. Cuesta;Tabet, Fatiha
目的：MicroRNA (miRNA) 在高密度脂蛋白 (HDL) 上运输，HDL 相关 miRNA 参与细胞间通讯。我们探讨了稳定和不稳定冠状动脉疾病患者冠状动脉循环中 HDL 相关 miRNA 浓度梯度，以及跨冠状动脉梯度的变化是否与 HDL 成分和大小的变化相关。 方法：对急性冠状动脉综合征 (ACS，n = 17) 患者、稳定型冠状动脉疾病患者 (稳定 CAD，n = 19) 和无 CAD 的对照受试者 (n = 6) 进行研究。 HDL 是从冠状窦 (CS)、主动脉根（动脉血）和右心房（静脉血）获得的血浆中分离出来的。 HDL 相关 miRNA（miR-16、miR-20a、miR-92a、miR-126、miR-222 和 miR-223）通过 TaqMan miRNA 测定进行定量。 HDL 粒径通过非变性聚丙烯酰胺梯度凝胶电泳测定。通过免疫比浊法或酶法测量 HDL 组成。结果：在冠状循环中观察到所有 HDL 相关 miRNA 的浓度梯度。在 ACS 患者中，与稳定 CAD 患者相比，HDL 相关 miR-16、miR-92a 和 miR-223 存在显着的逆冠状动脉梯度 (p < 0.05)。 HDL-miRNA 经冠状动脉梯度的变化与 HDL 组成或大小的变化无关。结论：与稳定 CAD 患者相比，ACS 患者经冠状动脉通道期间 HDL 中 miR-16、miR-92a 和 miR-223 被耗尽。 (c) 2017 Elsevier B.V. 保留所有权利。
Aims: MicroRNAs (miRNAs) are transported on high-density lipoproteins (HDLs) and HDL-associated miRNAs are involved in intercellular communication. We explored HDL-associated miRNAs concentration gradients across the coronary circulation in stable and unstable coronary artery disease patients and whether changes in the transcoronary gradient were associated with changes in HDL composition and size.Methods: Acute coronary syndrome (ACS, n = 17) patients, those with stable coronary artery disease (stable CAD, n = 19) and control subjects without CAD (n = 6) were studied. HDLs were isolated from plasma obtained from the coronary sinus (CS), aortic root (arterial blood) and right atrium (venous blood). HDL-associated miRNAs (miR-16, miR-20a, miR-92a, miR-126, miR-222 and miR-223) were quantified by TaqMan miRNA assays. HDL particle sizes were determined by non-denaturing polyacrylamide gradient gel electrophoresis. HDL composition was measured immunoturbidometrically or enzymatically.Results: A concentration gradient across the coronary circulation was observed for all the HDL-associated miRNAs. In ACS patients, there was a significant inverse transcoronary gradient for HDL-associated miR-16, miR-92a and miR-223 (p < 0.05) compared to patients with stable CAD. Changes in HDL-miRNA transcoronary gradients were not associated with changes in HDL composition or size.Conclusion: HDLs are depleted of miR-16, miR-92a and miR-223 during the transcoronary passage in patients with ACS compared to patients with stable CAD. (c) 2017 Elsevier B.V. All rights reserved.