INSULIN REGULATES INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA IN RAT HEPATOCYTES
INSULIN REGULATES INSULIN-LIKE GROWTH FACTOR-I MESSENGER-RNA IN RAT HEPATOCYTES
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DOI：
10.1152/ajpendo.1991.260.6.e846
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发表时间：
1991-06-01
影响因子：
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通讯作者：
FROESCH, ER
中科院分区：
文献类型：
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作者：
BONISCHNETZLER, M;SCHMID, C;FROESCH, ER
为了评估生长激素（GH）和胰岛素对胰岛素样生长因子I（IGF-I）mRNA水平的调节作用，我们采用原代大鼠肝细胞。 将细胞与10 nM胰岛素、10 nM GH或其组合孵育16 h，并通过北方印迹分析IGF-1 mRNA水平。 胰岛素导致IGF-I mRNA水平比无胰岛素对照组高2.5倍，GH导致IGF-I mRNA水平比无胰岛素对照组高3.8倍，胰岛素和GH的组合具有累加效应（6.7倍）。 在不同GH浓度下，10 nM胰岛素的作用是恒定的。 因此，GH和胰岛素相互独立地影响IGF-I mRNA水平。 胰岛素的半数最大有效剂量为4.7 × 10（-10）M，在动力学实验中，胰岛素在2小时内有效。 这些发现表明胰岛素通过直接调节IGF-I的转录水平来调节肝脏IGF-I的产生。
To evaluate the regulatory role of growth hormone (GH) and insulin on insulin-like growth factor I (IGF-I) mRNA levels, we employed primary rat hepatocytes. Cells were incubated for 16 h with 10 nM insulin, 10 nM GH, or a combination thereof, and IGF-I mRNA levels were analyzed by Northern blotting. Insulin results in 2.5-fold and GH in 3.8-fold higher IGF-I mRNA levels than hormone-free controls, and a combination of insulin and GH had an additive effect (6.7-fold). The effect of 10 nM insulin was constant at variable GH concentrations. Therefore, GH and insulin affect IGF-I mRNA levels independently of each other. The half-maximal effective dose of insulin was 4.7 x 10(-10) M, and, in kinetic experiments, insulin was effective within 2 h. These findings demonstrate that insulin modulates hepatic IGF-I production by a direct regulation of the transcript levels of IGF-I.