Association of Electrochemical Therapy With Optical, Mechanical, and Acoustic Impedance Properties of Porcine Skin
Association of Electrochemical Therapy With Optical, Mechanical, and Acoustic Impedance Properties of Porcine Skin
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DOI：
10.1001/jamafacial.2017.0341
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发表时间：
2017-11-01
影响因子：
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通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
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作者：
Moy, Wesley J.;Su, Erica;Wong, Brian J. F.
烧伤疤痕和其他皮肤损伤的传统治疗方法主要依赖于软组织移植，局部组织移植或植皮重新修复受损组织。利用针电极和电流在原位产生电化学反应可能是治疗疤痕和皮肤的新方法。目的研究电化学处理后猪皮肤的光学、力学和声阻抗特性的变化。2015年8月1日至2016年11月1日，研究了24个离体猪皮肤样本的局部ph驱动电化学治疗效果。将镀铂电极针插入新鲜猪皮样品中。直流电源提供4至5v的电压，3分钟的应用时间。使用光学相干断层扫描、光学相干弹性成像和超声成像对标本进行分析。超声成像3种（n = 2种），光学相干断层成像2种（n = 2种），光学相干弹性成像2种（n = 2种）。其余标本作为阳性对照组和阴性对照组（n = 10）。将镀铂电极针插入新鲜的猪皮肤样品中。直流电源提供4至5v的电压，使用时间为3分钟。在阳极和阴极观察到组织软化作为电化学修饰的结果。使用每种光学和声学技术记录体积变化。结果本初步研究共使用24个离体猪皮肤样本。光学相干断层扫描测量了每个电极周围表面组织变化的空间分布。在4v电压下持续3分钟，阳极和阴极的总体积效应分别为0.47 mm(3)和0.51 mm(3)。当电压为5 V，电压为3分钟时，阳极的总体积效应为0.85 mm(3)，阴极的总体积效应为1.05 mm(3)。结论与意义电化学治疗是一种低成本的技术，其成本与缝线和手术刀相当。利用电化学疗法在组织中产生机械和生理变化，有可能局部重塑软组织基质，最终可能导致廉价的疤痕治疗或皮肤年轻化治疗。
IMPORTANCE The classic management of burn scars and other injuries to the skin has largely relied on soft-tissue transfer to resurface damaged tissue with local tissue transfer or skin graft placement. In situ generation of electrochemical reactions using needle electrodes and an application of current may be a new approach to treat scars and skin.OBJECTIVE To examine the changes in optical, mechanical, and acoustic impedance properties in porcine skin after electrochemical therapy.DESIGN, SETTING, AND PARTICIPANTS This preclinical pilot study, performed from August 1, 2015, to November 1, 2016, investigated the effects of localized pH-driven electrochemical therapy of ex vivo porcine skin using 24 skin samples. Platinum-plated needle electrodes were inserted into fresh porcine skin samples. A DC power supply provided a voltage of 4 to 5 V with a 3-minute application time. Specimens were analyzed using optical coherence tomography, optical coherence elastography, and ultrasonography. Ultrasonography was performed under 3 conditions (n = 2 per condition), optical coherence tomography was performed under 2 conditions (n = 2 per condition), and optical coherence elastography was performed under 2 conditions (n = 2 per condition). The remaining samples were used for the positive and negative control groups (n = 10).EXPOSURES Platinum-plated needle electrodes were inserted into fresh porcine skin samples. A DC power supply provided a voltage of 4 to 5 V with a 3-minute application.MAIN OUTCOMES AND MEASURES Tissue softening was observed at the anode and cathode sites as a result of electrochemical modification. Volumetric changes were noted using each optical and acoustic technique.RESULTS A total of 24 ex vivo porcine skin samples were used for this pilot study. Optical coherence tomography measured spatial distribution of superficial tissue changes around each electrode site. At 4 V for 3 minutes, a total volumetric effect of 0.47 mm(3) was found at the anode site and 0.51 mm(3) at the cathode site. For 5 V for 3 minutes, a total volumetric effect of 0.85 mm(3) was found at the anode site and 1.05 mm(3) at the cathode site.CONCLUSIONS AND RELEVANCE Electrochemical therapy is a low-cost technique that is on par with the costs of suture and scalpel. The use of electrochemical therapy to create mechanical and physiologic changes in tissue has the potential to locally remodel the soft-tissue matrix, which ultimately may lead to an inexpensive scar treatment or skin rejuvenation therapy.