PDGF signaling from pharyngeal pouches promotes arch artery morphogenesis
PDGF signaling from pharyngeal pouches promotes arch artery morphogenesis
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来自咽袋的 PDGF 信号促进弓动脉形态发生
DOI：
10.1016/j.jgg.2019.11.004
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发表时间：
2019-12-20
影响因子：
5.9
通讯作者：
Wang, Qiang
中科院分区：
文献类型：
--
作者：
Mao, Aihua;Zhang, Mingming;Wang, Qiang
The great vessels of the heart originate from the pharyngeal arch arteries (PAAs). Anomalies of the PAAs often occur together with pharyngeal pouch malformations, but the reasons for this phenomenon are not fully understood. In the current study, we show that platelet-derived growth factor (PDGF) signaling derived from the pharyngeal pouches plays an important function in PAA vasculogenesis. During PAA development in zebrafish embryos, pdgf alpha a and pdgfab are expressed in the developing pharyngeal pouches. Results from loss-of-function experiments revealed a critical role of these genes in PAA formation. We found that nitroreductase (NTR)-mediated pouch ablation distinctly decreased PDGF receptor tyrosine phosphorylation, yielding a severe loss of PAAs. Importantly, pouch-specific overexpression of pdgf alpha a in pdgf alpha a(-/-); pdgf alpha b(-/-) mutants significantly relieved the PAA defects, which indicated a primary role of pharyngeal pouch-expressed PDGF ligands in signal activation and PAA morphogenesis. Our findings further showed that PDGF signaling was indispensable for the proliferation of PAA angioblasts. Together, these results established a role for PDGF alpha a- and PDGF alpha b-mediated tissue-tissue interaction during PAA development. Copyright (C) 2019, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
