Identification of a divergent actin-related protein in Drosophila.
Identification of a divergent actin-related protein in Drosophila.
复制标题
果蝇中不同肌动蛋白相关蛋白的鉴定。
DOI：
10.1006/jmbi.1994.1090
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发表时间：
1994
影响因子：
5.6
通讯作者：
Mooseker,MS
中科院分区：
文献类型：
--
作者：
Frankel,S;Heintzelman,MB;Artavanis-Tsakonas,S;Mooseker,MS
肌动蛋白相关蛋白（ARPs）与肌动蛋白具有一级序列同源性，与其他蛋白无同源性，与常规肌动蛋白不同，具有明显的差异性。我们已经在果蝇中发现了一个ARP，它与大多数肌动蛋白具有大约30%的氨基酸同源性，使其成为迄今为止报道的最多样化的。它与所有其他ARP序列也有很大的不同。当果蝇arp与肌动蛋白对齐时，它包含序列插入，就像所有其他arp一样。插入的独特位置，以及它的整体分化，表明它可能代表一个新的同型。与基因组DNA和多染色体杂交只检测到一个基因；基因的位置是在X染色体上的13E。在所有发育阶段检测到1350个碱基的转录本。该转录本在胚胎发生早期相对丰富，在胚胎发生后期减少，在幼虫和成虫中再次增加。
The actin-related proteins (ARPs) have primary sequence homology to actin, have no homology to other proteins and, unlike the conventional actins, are clearly divergent. We have identified an ARP inDrosophilathat has approximately 30% amino acid identity to most actins, making it the most divergent yet reported. It is also quite divergent from all other ARP sequences. When theDrosophilaARP is aligned with actin it contains sequence insertions, as is the case with all other ARPs. The unique location of the insertions, as well as its overall divergence, indicates it may represent a new isotype. Only one gene was detected by hybridization to both genomic DNA and polytene chromosomes; the location of the gene is 13E on the X chromosome. A transcript of 1350 bases was detected at all stages of development. This transcript was relatively abundant during early embryogenesis, decreasing during the later stages of embryogenesis and increasing again in larvae and adults.