Replication of a herpes-like virus in larvae of the flat oyster Tiostrea chilensis at ambient temperatures
Replication of a herpes-like virus in larvae of the flat oyster Tiostrea chilensis at ambient temperatures
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DOI：
10.3354/dao032161
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发表时间：
1998-04-03
影响因子：
1.4
通讯作者：
Besant, P
中科院分区：
文献类型：
--
作者：
Hine, PM;Wesney, B;Besant, P
在16至18摄氏度的温度下，从育雏的智利Tiostrea chilensis （Philippi， 1845年）中取出的幼虫在3至4天内的死亡率接近95%，这似乎与疱疹样病毒有关。去除后的噬菌体的超微结构观察显示，在4小时内存在早期病毒复制或假定的潜伏阶段，在24小时内所有阶段的复制都处于低水平，在48小时内增加到高水平，随后在72小时后死亡。最初，被感染的间质细胞或上皮细胞细胞核增大，呈波浪形轮廓，其中异染色质边缘。随着体积的不断增大，细胞核的轮廓变得光滑，异染色质减少或消失。透明核衣壳（LCC）和空衣壳出现在细胞核中，通常与直径约65nm的管状结构有关，这些管状结构由螺旋结构的亚基组成，其中包含直径约35nm的管状核。空衣壳和LCC有时出现在准晶阵列中。部分核仁解体和致密纤维物质的包膜先于包膜进入和离开核周池，在细胞质囊泡中形成和退出。部分感染细胞出现直径30 ~ 35nm的密集细胞质细丝群。显然，正常细胞在去除后4小时出现少量核内空衣壳和/或LCC可能代表潜伏性感染。在24 ~ 27℃的温度下，幼虫未观察到复制，但在48 h时，有少数细胞出现了大的低染色的核溶解核，并在其中观察到1 ~ 2个衣壳。病毒的复制与ranid herpesvirus 1 （Lucke肿瘤）感染相似。这是报告的第五个来自疱疹病毒的鸵鸟物种。
Veligers removed from brooding Tiostrea chilensis (Philippi, 1845) experienced similar to 95% mortalities over 3 to 4 d at 16 to 18 degrees C that appeared to be associated with a herpes-like virus. Ultrastructural observations of post-removal veligers showed the presence of early viral replication or putative latent stages at 4 h, all stages of replication at low levels at 24 h, which increased to high levels at 48 h, followed by mortalities at 72 h onwards. Initially, infected interstitial or epithelial cells had an enlarged nucleus with a wavy outline in which heterochromatin was marginated. With continued increase in size, nuclei became smooth in outline with reduction or loss of heterochromatin. Capsids with lucent cores (LCC) and empty capsids appeared in the nucleus, often in association with tubular structures similar to 65 nm in diameter that were composed of subunits in a helical configuration that contained a tubular core similar to 35 nm in diameter. Empty capsids and LCC sometimes occurred in paracrystalline arrays. Partial nucleolar disaggregation and encapsidation of dense fibrillar material preceded envelopment entering and de-envelopment leaving the perinuclear cisterna, tegumentation in cytoplasmic vesicles, and egress. Groups of dense cytoplasmic filaments 30 to 35 nm in diameter occurred in some infected cells. Apparently normal cells with a few intranuclear empty capsids and/or LCC at 4 h post-removal may represent latent infections. Replication was not observed in larvae held at 24 to 27 degrees C, but a few cells had enlarged hypochromatic karyolytic nuclei, and 1 to 2 capsids were observed in them, at 48 h. Viral replication was similar to that of ranid herpesvirus 1 (Lucke tumour) infections. This is the fifth ostreid species from which herpesviruses have been reported.