The Organization of the Cytoskeleton During Meiosis in Eggplant (Solanum Melongena (L.)): Microtubules and F-Actin are Both Necessary for Coordinated Meiotic Division
The Organization of the Cytoskeleton During Meiosis in Eggplant (Solanum Melongena (L.)): Microtubules and F-Actin are Both Necessary for Coordinated Meiotic Division
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茄子（Solanum Melongena (L.)）减数分裂过程中细胞骨架的组织：微管和 F-肌动蛋白对于协调减数分裂都是必需的
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
R. D. D. Vaulx
中科院分区：
文献类型：
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作者：
J. Traas;S. Burgain;R. D. D. Vaulx
由于两个分裂平面形成直角，高等植物的雄性减数分裂提供了分裂控制和空间规划的突出例子。为了研究这些过程中，我们染色微管和肌动蛋白丝在茄子雄性减数分裂。我们的研究结果表明：
(1)微管和它们的成核位点参与极性的建立;这得到了我们观察到的药物CIPC影响纺锤体极性的支持。(2)肌动蛋白微丝参与纺锤体的形成和完整性，但不参与极性的建立：细胞松弛素B和D影响纺锤体微管的组织，但不影响其极化分布。(3)在细胞分裂间期，从细胞两极的子核放射出来的微管可能通过将肌动蛋白集中在未来的分裂平面上而建立了未来的分裂平面（参见图1）。紫菀在动物细胞中定位收缩环的作用）。(4)在分裂平面上的这种浓度的F-肌动蛋白可能参与了胞质分裂的准备和对分裂平面的记忆（就像在极化组织中观察到的前期带一样）。(5)成膜体的形成是一个两步过程。中期I后不形成成膜体，中期II后形成四向成膜体，表明有丝分裂和胞质分裂不是强制偶联的。这些研究表明，肌动蛋白和微管共同参与的空间协调的分裂过程。
Address for reprints Because two division planes form at right angles, male meiosis in higher plants provides striking examples of both division control and spatial programming. To investigate these processes we have stained microtubules and actin filaments during male meiosis in the eggplant. Our results indicate the following.
(1) That microtubules and their nucleation sites are involved in the establishment of polarity; this is supported by our observation that the drug CIPC affects spindle polarity. (2) That actin microfilaments are involved in spindle formation and integrity, but not in the establishment of polarity: cytochalasin B and D affect the organization of the spindle microtubules, but not their polarized distribution. (3) That microtubules radiating from the daughter nuclei at the cell poles during interkinesis probably establish the future division plane by concentrating actin in that plane (cf. the proposed role of asters in positioning the contractile ring in animal cells). (4) That this concentration of F-actin in the division plane may be involved in preparing the cytoplasm for cytokinesis and in memorizing the division plane (much as the preprophase band observed in polarized tissues does). (5) That phragmoplast formation is a two-step process. No phragmoplast forms after metaphase I, but a four-way phragmoplast forms after metaphase II, indicating that mitosis and cytokinesis are not obligatorily coupled. These studies demonstrate that actin and microtubules are jointly involved in the spatial coordination of the division process.