Microfluidic cell electroporation using a mechanical valve
Microfluidic cell electroporation using a mechanical valve
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DOI：
10.1021/ac701854b
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发表时间：
2007-12-15
影响因子：
7.4
通讯作者：
Lu, Chang
中科院分区：
文献类型：
--
作者：
Wang, Jun;Stine, M. Jane;Lu, Chang
一个微流体电穿孔技术的基础上，证明了在聚二甲基硅氧烷（PDMS）制造的微芯片和一个共同的直流电源的弹性阀的操作。细胞膜透化所需的脉冲通过使用阀暂时中断回路来产生。用绿色DNA染料SYTOX对悬浮和贴壁的中国仓鼠卵巢细胞进行电透化。该技术消除了与脉冲发生器和微制造电极相关的成本和复杂性，这些电极通常涉及微尺度电穿孔装置。它还提供了集成电穿孔作为一个单元操作在大规模的微流体系统中的弹性阀在这些系统中的应用越来越多的潜力。
A microfluidic electroporation technique is demonstrated based on the operation of an elastomeric valve in a poly(dimethylsiloxane) (PDMS) fabricated microchip and a common dc power supply. The pulse needed for permeabilization of the cell membrane is generated by temporarily interrupting the circuit using the valve. The electropermeabilization of suspended and adherent Chinese hamster ovary cells with green DNA dye SYTOX is demonstrated. The technique eliminates the cost and complexity associated with a pulse generator and micro-fabricated electrodes that are often involved in microscale electroporation devices. It also offers the potential of integrating electroporation as a unit operation in large-scale microfluidic systems with the increasing application of elastomeric valves in these systems.