POSTNATAL OVARIAN FOLLICLE DEVELOPMENT IN HYPOGONADAL (HPG) AND NORMAL MICE AND ASSOCIATED CHANGES IN THE HYPOTHALAMIC PITUITARY OVARIAN AXIS
POSTNATAL OVARIAN FOLLICLE DEVELOPMENT IN HYPOGONADAL (HPG) AND NORMAL MICE AND ASSOCIATED CHANGES IN THE HYPOTHALAMIC PITUITARY OVARIAN AXIS
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DOI：
10.1530/jrf.0.0770287
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发表时间：
1986-05-01
期刊：
影响因子：
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通讯作者：
CHARLTON, HM
中科院分区：
文献类型：
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作者：
HALPIN, DMG;JONES, A;CHARLTON, HM
正常小鼠和性腺功能低下小鼠的子宫在第7天至第21天出现了明显的生长，但此后没有进一步的生长。在所有年龄，hpg小鼠的卵巢都明显小于正常小鼠，但在任何年龄，突变小鼠卵巢中不生长卵泡的数量与正常窝鼠之间没有显著差异，正常小鼠和hpg小鼠在出生后的第一个月里，不生长卵泡的数量明显减少。然而，hpg小鼠生长卵泡群的大小和组成与正常动物明显不同，到21日龄时，突变体的生长卵泡数量显著减少。在60日龄之前，3b型卵泡的数量没有显著差异，但在所有年龄的hpg小鼠中，所有其他类型的卵泡数量都明显减少。在突变体的卵巢中从未见过腔发育完全的卵泡（7型和8型），也从未见过黄体。hpg卵巢间质组织发育也很差。正常小鼠下丘脑GnRH含量一直保持在较低水平，直到第20天，然后急剧上升至成年水平（约为0.1%）。在第20天至第30天之间。垂体FSH含量在出生后的前10天上升，达到约5000 ng的峰值，然后在第30天降至约2000 ng的成体值，而血浆FSH浓度在出生后的前10天较高，但在接下来的20天降至成体水平。垂体LH含量在第5 ~ 10天显著升高，达到成虫600 ng左右的水平。在性腺功能低下的小鼠中，下丘脑GnRH在所有年龄段都检测不到，但垂体FSH和LH含量在第15天上升到衰减突变的成年值，并且与正常人不同，血浆FSH浓度在新生儿期没有升高。这些结果表明，从出生开始对卵巢进行最小程度的促性腺刺激对卵泡总数没有影响，但会减少正在生长的卵泡数量，并阻止卵泡生长超过早期心房期。因此，促性腺激素似乎在成年小鼠卵泡生长的开始和持续中起作用。
Significant uterine growth occurred in normal and hypogonadal (hpg) mice between Days 7 and 21 but thereafter no further growth was observed in hpg mice. The ovaries of hpg mice were significantly smaller than those of normals at all ages, but there was no significant difference between the number of non-growing follicles in the ovaries of mutants and their normal littermates at any age studied, and normal and hpg mice showed a marked reduction in the number of non-growing follicles during the first month of life. The size and composition of the growing follicle population in hpg mice, however, differed markedly from those in normal animals and by 21 days of age the number of growing follicles in mutants was significantly reduced. There was no significant difference in the number of Type 3b follicles before 60 days of age, but the number of all other follicles types was significantly less in hpg mice at all ages studied. Follicles in which the antrum is fully developed (Type 7 and 8) were never seen in the ovaries of mutants and corpora lutea were never observed. Interstitial tissue development was also very poor in hpg ovaries. The hypothalamic GnRH content in normal mice remained low until Day 20, before rising sharply to adulte levels (.apprx. 800 pg) between Days 20 and 30. The pituitary FSH content increased over the first 10 days of life to reach a peak of about 5000 ng, before declining to the adult value of about 2000 ng by Day 30, while the plasma FSH concentration was high in the first 10 days, but fell to adult levels over the next 20 days. Pituitary LH content increased significantly between Days 5 and 10 to reach the adult level of about 600 ng. Hypothalamic GnRH was undetectable at all ages in hypogonadal mice, but the pituitary content of FSH and LH had risen to the attenuated mutant adult value by Day 15, and unlike normals, plasma FSH concentrations were not elevated during the neonatal period. These results suggest that minimal gonadotrophic stimulation of the ovary from birth has no effect on the total number of follicles but reduces the number of growing follicles and prevents follicle growth beyond the early antral stage. Gonadotrophins therefore appear to have a role in the initiation and continuance of follicle growth in the adult mouse.