Polysomal RNA in Semliki Forest virus infected Aedes albopictus cells.

Polysomal RNA in Semliki Forest virus infected Aedes albopictus cells.
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塞姆利基森林病毒感染白纹伊蚊细胞中的多聚体 RNA。

DOI:
10.1099/0022-1317-29-1-35
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发表时间:
1975
期刊:
The Journal of general virology
影响因子:
--
通讯作者:
R. Regnery
R. Regnery
中科院分区:
--
文献类型:
--
作者:
B. Eaton;R. Regnery

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Polysomes from Aedes albopictus cells were identified by their rapid labelling with radioactive amino acids and their sensitivity to EDTA, RNase and puromycin. The major ribosome component in cytoplasmic extracts had a sedimentation coefficient of approx. 95S and may be a ribosome dimer. In Semiki Forest virus infected Aedes albopictus cells, 42S and 26S RNA were the major virus RNA species detected up to 10 h post infection. Virus RNA was detected in association with pre-labelled ribosomes and banded at a buoyant density of 1-55 g/ml. 42S, 38S and 26S virus RNA species were associated with polysomes.