Potassium and taurine release are highly correlated with regulatory volume decrease in neonatal primary rat astrocyte cultures.
Potassium and taurine release are highly correlated with regulatory volume decrease in neonatal primary rat astrocyte cultures.
复制标题
钾和牛磺酸的释放与新生原代大鼠星形胶质细胞培养物中调节体积的减少高度相关。
DOI：
10.1046/j.1471-4159.1994.63031143.x
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发表时间：
1994
影响因子：
4.7
通讯作者：
Aschner,M
中科院分区：
文献类型：
--
作者：
Vitarella,D;DiRisio,DJ;Kimelberg,HK;Aschner,M
Neonatal rat primary astrocyte cultures were swollen by exposure to hypotonic buffer. Using an electrical impedance method for determination of cell volume coupled with on‐line measurements of efflux of radioactive ions or amino acids, we have investigated the role of K+(using86Rb), taurine, andd‐aspartate (an analogue of glutamate) in regulatory volume decrease (RVD). Addition of 1 mMquinine, 10 µMnimodipine, 100 µMBAPTA‐AM, 10 µMtrifluoperazine, or a calcium‐free buffer significantly (p< 0.0001) inhibited RVD. This was accompanied by inhibition of86Rb release but an increase ind‐[3H]‐aspartate release, which was proportional to the degree to which RVD was inhibited. These results support a regulatory role for calcium in RVD and show that inhibition of calcium entry from the extracellular fluid, intracellular calcium sequestration, inhibition of calcium‐activated K+channels, and inhibition of calmodulin all inhibit RVD. Becaused‐[3H]aspartate efflux profiles increase as RVD is inhibited, it is unlikely thatd‐aspartate release is a main determinant of RVD. In contrast, [3H]taurine release was increased by 1 mMquinine and inhibited by 10 µMtrifluoperazine. The net release of K+and taurine is highly correlated with the degree of RVD, implicating a regulatory role for both K+and taurine release in RVD.
