ENCAPSULATION OF PORCINE SPERMATOZOA IN POLYLYSINE MICROSPHERES
ENCAPSULATION OF PORCINE SPERMATOZOA IN POLYLYSINE MICROSPHERES
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DOI：
10.1016/0093-691x(90)90507-p
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发表时间：
1990-02-01
期刊：
影响因子：
2.8
通讯作者：
NEBEL, RL
中科院分区：
文献类型：
--
作者：
ESBENSHADE, KL;NEBEL, RL
进行了三个实验，以检查在浓缩样品中孵育猪精子的效果，以确定微球包封的精子的活力，并评估微囊化猪精子用于人工授精的潜力。在实验1中，精子在4、15、20或37度下孵育。浓度为7.5、15、30、60或120 × 10 - 6。在16小时的孵育期内，105个精子/ml丧失活力。精子活力在4.0 ℃时显著降低。 在15 ℃、20 ℃或37 ℃时，C和显着更高的浓度样品。在实验2中，将精子包封在浓度为30、60或120 × 10 - 6的聚赖氨酸微球中。106精子/ml，并在4、15或20 ℃下体外孵育。C.将未包封的样品在相似的浓度和温度下孵育，并作为对照。在孵育2、4、8和16 h时，估计顶体完整的精子活力和百分比。包封过程不影响精子活力或顶体形态;然而，包封样品的活力加速丧失。两组之间的顶体形态在时间上无差异。在实验3中，精子以120 × 104的浓度被包囊。用106个精子/ml和20 ml胶囊输注发情母猪。在授精后3、6和24 h冲洗子宫内容物，并检查胶囊。在3和6 h时从母猪中回收了含有活动精子的胶囊，但在24 h时未回收。这些结果表明，猪精子可以被封装在微球中，这些胶囊可以授精到发情期的女性，但精子经历在体外和体内的运动力的加速损失。
Three experiments were conducted to examine the effects of incubating porcine spermatozoa in concentrated samples, to determine the viability of sperm encapsulated in microspheres and to evaluate the potential of microencapsulating porcine spermatozoa for use in artificial insemination. In Experiment 1, sperm incubated at 4, 15, 20 or 37.degree. C and at concentrations of 7.5, 15, 30, 60 or 120 .times. 105 sperm/ml lost motility over a 16-h incubation period. Sperm motility was significantly lower at 4.degree. C than at 15, 20 or 37.degree. C and was significantly higher in more concentrated samples. In Experiment 2, sperm were encapsulated in poly-lysine microspheres at concentrations of 30, 60 or 120 .times. 106 sperm/ml and incubated in vitro at 4, 15 or 20.degree. C. Unencapsulated samples were incubated at similar concentrations and temperature and served as controls. Motility and percentage of sperm with intact acrosomes were estimated at 2, 4, 8 and 16 h of incubation. The procedure of encapulation did not affect sperm motility or acrosomal morphology; however, there was an accelerated loss of motility in encapsulated samples. There were no differences in acrosomal morphology between the two groups across time. In Experiment 3, sperm were encapsulated at a concentration of 120 .times. 106 sperm/ml and 20 ml of capsules were inseminated into estrous sows. Uterine contents were flushed at 3, 6 and 24 h after insemination and examined for capsules. Capsules containing motile sperm were recovered from sows at 3 and 6 h, but not at 24 h. These results demonstrate that porcine spermatozoa can be encapsulated in microspheres and that these capsules can be inseminated into estrous females, but the sperm undergo an accelerated loss of motility in vitro and in vivo.