Trigeminal-parabrachial connections: Possible pathway for nociception-induced cardiovascular reflex responses
Trigeminal-parabrachial connections: Possible pathway for nociception-induced cardiovascular reflex responses
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DOI：
10.1016/0006-8993(95)01580-9
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发表时间：
1996-04-09
期刊：
影响因子：
2.9
通讯作者：
Esser, MJ
中科院分区：
文献类型：
--
作者：
Allen, GV;Barbrick, B;Esser, MJ
神经刺激牙神经可引起心血管功能的显著变化。为了研究中枢通路介导的心血管活动的反射变化，免疫组织化学定位的细胞表达的立即早期基因，c-Sos，被用来识别中枢神经元响应于伤害性电刺激的下颌，切牙牙本质或化学（辣椒素）刺激的牙髓在麻醉大鼠。在臂旁外侧区注射Fluoro-Gold以确定三叉神经脊束核的传出投射。电刺激和化学刺激在三叉神经脊束核中产生类似的Fos阳性神经元模式：尾侧亚核、极间亚核和口侧亚核。Fos阳性神经元主要分布在尾侧背内侧亚核的I层和LT层，极间亚核和口侧亚核的Fos阳性神经元较少。假刺激牙本质和控制车辆注射到牙髓导致在少数弱染色或没有Fos阳性神经元的三叉神经脊束核。细胞体双标记的Fluoro-Gold注射到臂旁区和Fos蛋白电刺激后的切牙存在于三叉神经脊束核的所有三个亚核。向臂旁外侧区传出投射的Fos阳性神经元最多位于尾侧亚核（32.2 ± 5.3 S.E.M.）。位于极间区（0.4 ± 0.4S.E.M.）和口腔（19.8 +/- 3.5 S.E.M.）亚核结果表明，三叉神经脊束核的三个亚核，特别是尾侧亚核，接受的伤害性牙齿输入，被中继到臂旁外侧核。
Noxious stimulation of dental nerves elicits marked changes in cardiovascular function. In order to investigate central pathways mediating reflex changes in cardiovascular activity, immunohistochemical localization of cells expressing the immediate-early gene, c-Sos, was used to identify central neurons responding to noxious electrical stimulation of mandibular, incisor tooth dentin or chemical (capsaicin) stimulation of tooth pulp in the anesthetized rat. Injections of Fluoro-Gold were made in the lateral parabrachial region to identify efferent projections from the spinal trigeminal nucleus. Electrical and chemical stimulation produced similar patterns of Fos-positive neurons in the spinal trigeminal nucleus: subnuclei caudalis, interpolaris and oralis. Fos-positive neurons were most dense in laminae I and LT, of the dorsomedial subnucleus caudalis with fewer Fos-positive neurons located in the interpolaris and oralis subnuclei. Sham stimulation of tooth dentin and control vehicle injections into the tooth pulp resulted in either a few weakly stained or no Fos-positive neurons in the spinal trigeminal nucleus. Cell bodies double labeled with Fluoro-Gold following injections into the parabrachial region and Fos-protein subsequent to electrical stimulation of incisor tooth were present in all three subnuclei of the spinal trigeminal nucleus. The largest number of Fos-positive neurons with efferent projections to the lateral parabrachial region were located in subnucleus caudalis (32.2 +/- 5.3 S.E.M.) and fewer were located in the interpolaris (0.4 +/- 0.4 S.E.M.) and oralis (19.8 +/- 3.5 S.E.M.) subnuclei. The results demonstrate that nociceptive dental input received by the three subnuclei of the spinal trigeminal nucleus, particularly the subnucleus caudalis, is relayed to the lateral parabrachial nucleus.