In Situ Growth of Surfactant-Free Gold Nanoparticles on Nitrogen-Doped Graphene Quantum Dots for Electrochemical Detection of Hydrogen Peroxide in Biological Environments
In Situ Growth of Surfactant-Free Gold Nanoparticles on Nitrogen-Doped Graphene Quantum Dots for Electrochemical Detection of Hydrogen Peroxide in Biological Environments
复制标题
无表面活性剂金纳米颗粒在氮掺杂石墨烯量子点上的原位生长用于生物环境中过氧化氢的电化学检测
DOI：
10.1021/ac5041555
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发表时间：
2015-02-03
影响因子：
7.4
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Ju, Jian;Chen, Wei
在这项工作中，我们报告了一种绿色和简单的策略，用于在氮掺杂石墨烯量子点（Au NPs- n- gqds）上原位生长无表面活性剂的Au纳米颗粒（Au NPs）。在不添加任何还原剂和表面活性剂的情况下，仅将N-GQDs与HAuCl4中心点4H(2)O混合即可形成杂化产物。高分辨率透射电镜（HRTEM）、x射线衍射（XRD）和x射线光电子能谱（XPS）表征清楚地表明，形成的Au纳米颗粒具有主要暴露的（111）面，可以提供更多的吸附位点。这种非表面活性剂包覆的Au NPs可以提供具有高电催化活性的裸催化表面。Au NPs-N-GQDs对过氧化氢（H2O2）的电化学检测具有较高的灵敏度和选择性，检出限为0.12 μ M，灵敏度为186.22 μ a /mM cm(2)。重要的是，基于Au nps - n - gqds的电化学生物传感器在检测人血清样品和人宫颈癌细胞释放的H2O2水平方面显示出巨大的应用潜力，并取得了令人满意的结果。本研究表明，这种新型Au NPs-N-GQDs纳米复合材料有望用于制备非酶促H2O2生物传感器。
In this work, we report a green and simple strategy for the in situ growth of surfactant-free Au nanoparticles (Au NPs) on nitrogen-doped graphene quantum dots (Au NPs-N-GQDs). The formation of hybrid was achieved by just mixing the N-GQDs and HAuCl4 center dot 4H(2)O without addition of any other reductant and surfactant. High-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) characterizations clearly showed the formation of Au nanoparticles with predominantly exposed (111) facets which can provide more adsorption sites. Such nonsurfactant-capped Au NPs can provide naked catalytic surface with highly electrocatalytic activity. The Au NPs-N-GQDs exhibit high sensitivity and selectivity for electrochemical detection of hydrogen peroxide (H2O2) with a low detection limit of 0.12 mu M and sensitivity of 186.22 mu A/mM cm(2). Importantly, the Au NPs-N-GQDs-based electrochemical biosensor has shown great potential applications for detection of H2O2 levels in human serum samples and that released from human cervical cancer cells with satisfactory results. The present study demonstrates that such novel Au NPs-N-GQDs nanocomposite is promising for fabrication of nonenzymatic H2O2 biosensors.