Genetic manipulation of proline levels affects antioxidants in soybean subjected to simultaneous drought and heat stresses
Genetic manipulation of proline levels affects antioxidants in soybean subjected to simultaneous drought and heat stresses
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DOI：
10.1111/j.1399-3054.2005.00504.x
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发表时间：
2005-06-01
影响因子：
6.4
通讯作者：
de Ronde, JA
中科院分区：
文献类型：
--
作者：
Kocsy, G;Laurie, R;de Ronde, JA
据推测，由于两种化合物都有共同的前体谷氨酸，因此对Pro水平的遗传操作也会影响(高)谷胱甘肽的含量。为了验证这一假设，在时间进程实验中，比较了同时干旱和热胁迫条件下和对照处理下野生型大豆(Glyinemax cv.)Pro、还原型和氧化型谷胱甘肽[(H)GSH和(H)GSSG]和其他抗氧化剂的水平。IBIS)和含有L-Delta(1)-吡咯烷-5-羧酸还原酶(EC1.5.1.2)的转基因植株，EC1.5.1.2是Pro合成的最后一个酶，正向和反义方向。在恢复期结束时，反义转化子的过氧化氢和脂质过氧化氢浓度最高，表现出最大的伤害，而正义转化子中的过氧化氢含量最低，损伤百分率最低。在胁迫处理过程中，反义转化子中的Pro和抗坏血酸(AA)含量最高，而GSH和hGSH含量、AA/脱氢抗坏血酸(DHA)和(H)GSH/(H)GSSG比值以及抗坏血酸过氧化物酶(APX)活性最高。反义转化子的APX(EC 1.11.1.11)活性在胁迫处理的第一部分达到最大，谷胱甘肽还原酶(EC 1.6.4.2)的活性在处理后的第二部分达到最大。目前的实验表明，Pro合成的操纵不仅影响(H)GSH浓度，而且还影响其他抗氧化剂的水平。
It was assumed that the genetic manipulation of the proline (Pro) level would also affect the (homo)glutathione content as both compounds have a common precursor, glutamate. To test this hypothesis, the levels of Pro, reduced and oxidized (homo)glutathione [(h)GSH and (h)GSSG] and other antioxidants were compared under simultaneous drought and heat stress conditions and in a control treatment in a time course experiment on wild-type soybean (Glycine max cv. Ibis) and on transgenic plants containing the cDNA coding for L-Delta(1)-pyrroline-5-carboxylate reductase (EC 1.5.1.2), the last enzyme involved in Pro synthesis, in the sense and antisense directions. At the end of the recovery period, the highest H2O2 and lipid hydroperoxide concentrations were observed in the antisense transformants, which exhibited the greatest injury, while the lowest H2O2 content was detected in the sense transformants, which exhibited the lowest injury percentage. During stress treatment, the highest Pro and ascorbate (AA) levels were detected in the sense transformants, while the highest GSH and hGSH contents, AA/dehydroascorbate (DHA) and (h)GSH/(h)GSSG ratios and ascorbate peroxidase (APX) activity were found in the antisense transformants. The greatest APX (EC 1.11.1.11) activity was observed in the first part of the stress treatment in the antisense transformants, and the greatest glutathione reductase (EC 1.6.4.2) activity was observed in the second part of the treatment in the same genotype. The present experiments indicate that the manipulation of Pro synthesis affects not only the (h)GSH concentrations, but also the levels of other antioxidants.