SPECIFICITY AND LOCALIZATION OF LIPOLYTIC-ACTIVITY IN ADULT DROSOPHILA-MELANOGASTER
SPECIFICITY AND LOCALIZATION OF LIPOLYTIC-ACTIVITY IN ADULT DROSOPHILA-MELANOGASTER
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DOI：
10.1042/bj3040775
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发表时间：
1994-12-15
影响因子：
4.1
通讯作者：
BOWNES, M
中科院分区：
文献类型：
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作者：
SMITH, GM;ROTHWELL, K;BOWNES, M
研究了黑腹果蝇成虫体内存在的甘油三酯脂肪酶。果蝇的不同组织中存在不同的脂肪酶活性。特别是，在男性副腺中存在丰富的脂肪酶活性。用酯酶零突变体证实该酶活性是由一种独特的脂肪酶引起的，而不是由副腺体中也丰富的酯酶6引起的非特异性活性。研究了副腺脂肪酶的性质，pH值和底物利用表明它与脊椎动物胆盐刺激脂肪酶有一些相似之处。脂肪酶活性在雄性中显著降低，雌性在交配后不久增加。这一发现表明，脂肪酶活性转移到雌性，可能在果蝇的交配和繁殖中起重要作用。
The triacylglycerol lipases present in adult Drosophila melanogaster have been investigated. Different lipase activities are present in various tissues in the fly. In particular, an abundant lipase activity is present in the male accessory gland. An esterase null mutant was used to confirm that the enzyme activity was due to a distinct lipase and not non-specific activity from esterase 6 which is also abundant in accessory glands. The properties of the accessory-gland lipase were investigated, and pH optima and substrate utilization suggest that it has some similarities to vertebrate bile-salt-stimulated lipase. Lipase activity is significantly reduced in males and increased in females shortly after mating. This finding suggests that lipase activity is transferred to the female and may be important in mating and reproduction in Drosophila.