Overexpression of rice aquaporin OsPIP1;2 improves yield by enhancing mesophyll CO2 conductance and phloem sucrose transport
Overexpression of rice aquaporin OsPIP1;2 improves yield by enhancing mesophyll CO2 conductance and phloem sucrose transport
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水稻水通道蛋白 OsPIP1;2 的过度表达通过增强叶肉 CO2 电导和韧皮部蔗糖运输来提高产量
DOI：
10.1093/jxb/ery386
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发表时间：
2018-12
影响因子：
6.9
通讯作者：
Yiyong Zhu
中科院分区：
文献类型：
--
作者：
Feiyun Xu;Ke Wang;Wei Yuan;Weifeng Xu;Shuang Liu;Herbert J Kronzucker;Guanglei Chen;Rui Miao;Maoxing Zhang;Ming Ding;Liang Xiao;Lei Kai;Jianhua Zhang;Yiyong Zhu
水通道蛋白参与CO2从叶片细胞间隙向叶绿体的运输，这有助于CO2同化。然而，水稻（Oryza sativa L.）水通道蛋白是未知的。在这里，我们研究了水通道蛋白OsPIP 1;2在CO2扩散相关的光合作用和韧皮部蔗糖运输的功能。此外，测定过表达OsPIP 1;2的水稻品系的谷粒产量。OsPIP1; 2定位于质膜和OsPIP 1的相对表达量;2是约5倍高的CO2浓度升高的存在下，在叶片中。与野生型（WT）水稻相比，OsPIP 1;2的过表达使叶肉导度增加约150%。OsPIP 1;2过表达株系的生物量高于野生型，这可能是由于韧皮部蔗糖转运增加所致。此外，在三季田间试验中，OsPIP 1;2过表达株系的籽粒产量比WT高约25%，这是由于有效分蘖数和每穗颖花数增加。我们的研究结果表明OsPIP 1;2通过促进叶片CO2扩散，增加CO2净同化率和蔗糖转运，调节水稻生长和产量
Aquaporins are involved in CO2 transport from the leaf intercellular air space to the chloroplast, which contributes to CO2 assimilation. However, the mechanism of CO2 transport by rice (Oryza sativa L.) aquaporins is unknown. Here, we investigated the function of the aquaporin OsPIP1;2 in CO2 diffusion-associated photosynthesis and phloem sucrose transport. Moreover, the grain yield of rice lines overexpressing OsPIP1;2 was determined. OsPIP1;2 was localized to the plasma membrane and the relative expression of OsPIP1;2 was approximately 5-fold higher in leaves in the presence of an elevated CO2 concentration. Overexpression of OsPIP1;2 increased mesophyll conductance by approximately 150% compared with wild-type (WT) rice. The OsPIP1;2-overexpressing lines had higher biomass than the WT, possibly due to increased phloem sucrose transport. In addition, the grain yield of OsPIP1;2-overexpressing lines was approximately 25% higher than that of the WT in three-season field experiments, due to the increased numbers of effective tillers and spikelets per panicle. Our results suggest that OsPIP1;2 modulates rice growth and grain yield by facilitating leaf CO2 diffusion, which increases both the net CO2 assimilation rate and sucrose transport
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
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通讯作者：
Jones, Hamlyn G.
影响因子：
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作者：
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通讯作者：
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