The cartilaginous fracture callus in rats.
The cartilaginous fracture callus in rats.
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DOI：
10.3109/17453678709146476
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发表时间：
1987-06
期刊：
影响因子：
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通讯作者：
A. Henricson;A. Hulth;O. Johnell
中科院分区：
文献类型：
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作者：
A. Henricson;A. Hulth;O. Johnell
100 g大鼠56只，手工断右胫骨；骨折用髓内钢丝固定。各组大鼠于3 ~ 30 d后处死。将骨折及其周围的肌肉组织解剖出来，并立即冷冻至-70℃。骨折区域的低温切片用苏木精/伊红、甲苯胺蓝染色，并用II型胶原抗体免疫酶法染色。另一组30只骨折大鼠于1-15天后处死，用EDTA脱钙后观察骨折的组织学变化。观察到两种类型的愈伤组织。第1天，骨膜-骨内膜愈伤组织以无炎症细胞的前成骨细胞增生开始，第3天至第8-10天形成骨小梁，此后不再形成骨小梁。软骨愈伤组织由成纤维细胞样间充质细胞与骨膜愈伤组织外的炎症细胞混合凝结形成，并于第5天在骨膜剥离的骨折碎片处开始形成。分化成软骨的细胞似乎是从周围的肌肉组织及其新形成的血管中迁移过来的。第11天，内软骨骨在骨膜愈伤组织附近开始形成，血管从骨膜愈伤组织穿入当时肥大和矿化的软骨细胞。我们认为桥接性愈伤组织是由来源于预定细胞的骨膜愈伤组织的基础和由外部细胞迁移形成的内软骨骨小梁的桥接形成的。
The right tibia was broken manually in 56 rats weighing 100 g; the fracture was stabilized with an intramedullary steel wire. Groups of rats were killed after 3-30 days. The fracture with its surrounding musculature was dissected out and immediately frozen to -70 degrees C. Cryostat sections of the fracture region were stained with hematoxylin/eosin, toluidine blue, and immunoenzymatically with collagen II antibodies. Another series of 30 fractured rats were killed after 1-15 days, and the fractures were examined histologically after decalcification with EDTA. Two types of callus were observed. The periosteal-endosteal callus started as proliferation of pre-osteoblasts without inflammatory cells on Day 1 and developed bone trabeculae from Day 3 until Days 8-10, but not thereafter. The cartilaginous callus was formed by condensation of fibroblast-like mesenchymal cells mixed with inflammatory cells outside the periosteal callus and started on Day 5 at the fracture fragments denuded of periosteum. The cells differentiating to cartilage seemed to migrate from the surrounding musculature and its newly formed vessels. The enchondral bone formation started close to the periosteal callus from which vessels were piercing into the then hypertrophic and mineralized chondrocytes on Day 11. We conclude that the bridging callus is formed by fundamentals of periosteal callus derived from predetermined cells and the bridge of enchondrally formed bone trabeculae by cells migrating from outside.