Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy
Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy
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DOI：
10.1002/jbio.200910062
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发表时间：
2010-01-01
影响因子：
2.8
通讯作者：
Pavone, Francesco S.
中科院分区：
文献类型：
--
作者：
Cicchi, Riccardo;Kapsokalyvas, Dimitrios;Pavone, Francesco S.
我们已经使用非线性成像来评估结缔组织离体样品中的胶原组织。图像分析方法在健康真皮、正常疤痕和瘢痕疙瘩上进行了测试。真皮的二次谐波到自体荧光老化指数（SAAID）的评估允许通过评分胶原/弹性蛋白含量来对组织进行首次表征。通过SHG图像模式分析，进一步分析了健康真皮和瘢痕疙瘩的胶原形态。灰度共生矩阵（GLCM）分析方法可以根据胶原纤维束的几何排列进行不同组织的分类，而FFT图像的模式分析可以根据胶原纤维分布的各向异性来区分不同的组织，这种多重评分方法代表了一种有希望扩展到其他胶原疾病的工具。也可用于体内皮肤成像应用。【图片】瘢痕疙瘩切片，H&E染色，非线性显微镜成像。视场：1 x 0.5 mm (C) 2010由WILEY-VCH Verlag GmbH & Co. KGaA。Weinheim
We have used nonlinear imaging to evaluate collagen organization in connective tissue ex-vivo samples. Image analysis methods were tested on healthy dermis, normal scars, and keloids. The evaluation of the second harmonic to autofluorescence aging index of dermis (SAAID) has allowed a first characterization of tissues by scoring the collagen/elastin content. Further analyses on collagen morphology in healthy dermis and keloids were performed by image-pattern analysis of SHG images. The gray-level co-occurrence matrix (GLCM) analysis method has allowed classification of different tissues based on the evaluation of geometrical arrangement of collagen fibrillar bundles, whereas a pattern analysis of the FFT images has allowed the discrimination of different tissues based on the anisotropy of collagen fibers distribution, This multiple scoring method represents a promising tool to be extended to other collagen disorders, as well as to be used in in-vivo skin-imaging applications.[GRAPHICS]Slice of a sample of keloid labeled with H&E staining and imaged using nonlinear microscopy. Field of view: 1 x 0.5 mm (C) 2010 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim