NEK2 regulates stem-like properties and predicts poor prognosis in hepatocellular carcinoma
NEK2 regulates stem-like properties and predicts poor prognosis in hepatocellular carcinoma
复制标题
DOI：
10.3892/or.2016.4896
复制
发表时间：
2016-08-01
期刊：
影响因子：
4.2
通讯作者：
Cai, Xiujun
中科院分区：
文献类型：
--
作者：
Lin, Shuang;Zhou, Senjun;Cai, Xiujun
据估计，NEK2在癌症进展中起着重要作用。然而，其与肝细胞癌（HCC）的相关性尚未被探讨。免疫组化显示NEK2在HCC中表达上调。Kaplan-Meier曲线显示nek2阳性肝细胞癌患者术后预后较nek2阴性患者差。NEK2的缺失降低了细胞系的自我更新特性和化疗耐药性，并减少了干细胞相关基因。NEK2与HCC患者的不良预后相关，并通过Wnt/ β -catenin信号传导调节自我更新特性，并通过优先调节HCC细胞中ABCG2和ALDH1A1的表达来调节化疗耐药。
NEK2 has been estimated to play an important role in cancer progression. However, its relevance in hepatocellular carcinoma (HCC) has not yet been explored. Immunohistochemistry revealed NEK2 expression was upregulated in HCC. NEK2-positive hepatocellular carcinoma patients were associated with poor prognosis after surgery compared with NEK2-negative patients based on Kaplan-Meier curves. Deletion of NEK2 reduced self-renewal properties and chemotherapeutic resistance, and decreased the stemness associated genes in cell lines. NEK2 was associated with unfavorable outcomes in HCC patients, and was revealed to regulate self-renewal property by means of Wnt/beta-catenin signaling, and chemotherapeutic resistance by preferential regulation of the expression of ABCG2 and ALDH1A1 in HCC cells.