Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A
Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A
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DOI：
10.1007/s10529-008-9718-1
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发表时间：
2008-09-01
影响因子：
2.7
通讯作者：
Proft, Thomas
中科院分区：
文献类型：
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作者：
Clow, Fiona;Fraser, John D.;Proft, Thomas
由于对基于蛋白质的传感器的强烈兴趣，蛋白质表面的固定化是一个活跃的研究领域。在这里，我们描述了一种利用金黄色葡萄球菌分类酶a （SrtA）的转肽酶活性将配体蛋白固定在Biacore传感器芯片上的新方法。这种方法为蛋白质与生物传感器芯片的位点导向共价偶联提供了一种稳健而温和的方法。值得注意的是，该分选酶的高特异性允许从非纯蛋白质样品中固定蛋白质，从而缩短了蛋白质纯化方案。
The immobilization of proteins to surfaces is an active area of research due to strong interest in protein-based sensors. Here, we describe a novel method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A (SrtA). This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. Notably, the high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols.