Urinary metabonomic study using a CUMS rat model of depression
Urinary metabonomic study using a CUMS rat model of depression
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DOI：
10.1002/mrc.2865
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发表时间：
2012-03-01
影响因子：
2
通讯作者：
Qin, Xue-Mei
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Jie;Li, Zhen-Yu;Qin, Xue-Mei
慢性不可预测的轻度应激（CUMS）是一种经过充分验证的抑郁症模型。本研究采用基于核磁共振波谱测定的尿液代谢组学方法来研究 CUMS 诱导的大鼠抑郁模型中的代谢扰动。通过模式识别分析，实现了 CUMS 大鼠和健康对照的清晰分离，并鉴定了有助于分离的九种内源代谢物。 CUMS治疗的大鼠的特点是甘氨酸、丙酮酸、谷氨酰胺和天冬酰胺增加，以及2-酮戊二酸、二甲基甘氨酸、柠檬酸、琥珀酸和乙酸盐减少。讨论了CUMS诱发抑郁症时与代谢紊乱相关的尿液生化变化，以及与中医活气郁证可能的相关性。这项工作表明，CUMS 是研究抑郁症的可靠模型，无创尿代谢组学方法是研究抑郁症生化扰动作为早期诊断手段的宝贵工具。版权所有 (C) 2012 约翰·威利父子有限公司
Chronic unpredictable mild stress (CUMS) is a well-validated model of depression. In this study, a urinary metabonomics method based on the NMR spectrometry was used to study the metabolic perturbation in CUMS-induced rat depression model. With pattern recognition analysis, a clear separation of CUMS rats and healthy controls was achieved, and nine endogenous metabolites contributing to the separation were identified. CUMS-treated rats were characterized by the increase of glycine, pyruvate, glutamine, and asparagines, as well as the decrease of 2-oxoglutarate, dimethylglycine, citrate, succinate, and acetate. The urinary biochemical changes related to the metabolic disturbance in CUMS induced depression, and the possible correlations with live qi stagnation in traditional Chinese medicine are discussed. The work shows that CUMS is a reliable model for studying depression, and the noninvasive urinary metabolomic method is a valuable tool to investigate the biochemical pertubations in depression as an early diagnostic means. Copyright (C) 2012 John Wiley & Sons, Ltd.