A Cre-Reporter Transgenic Mouse Expressing the Far-Red Fluorescent Protein Katushka
A Cre-Reporter Transgenic Mouse Expressing the Far-Red Fluorescent Protein Katushka
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DOI：
10.1002/dvg.20685
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发表时间：
2011-01-01
期刊：
影响因子：
1.5
通讯作者：
Ortega, Sagrario
中科院分区：
文献类型：
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作者：
Dieguez-Hurtado, Rodrigo;Martin, Javier;Ortega, Sagrario
Cre/IoxP依赖的荧光蛋白表达代表了用于细胞谱系、命运作图和遗传分析的强大生物学工具。随着远红荧光蛋白的发展，活体组织成像得到了显着改善，并具有针对体内应用的优化光谱特性。在这里，我们报告的第一代转基因小鼠系表达远红荧光蛋白Katushka，驱动的混合CAG启动子后，铬介导的重组。在生殖系或组织特异性Cre驱动的报告基因激活后，Katushka表达强烈且普遍存在，没有毒性作用，允许在来自检查的所有组织的新鲜和固定样品中进行荧光检测。此外，当Katuhska仅在动物体内深处的特定细胞群（如胰腺β细胞）中表达时，可以通过体内非侵入性全身成像检测荧光。因此，这种报告模型能够早期，广泛和敏感的Cre活性的体内检测，并应提供一个通用的工具，广泛的荧光和实时成像应用。创世纪49：36-45，2011。(C)2010 Wiley-Liss，Inc.
Cre/IoxP-dependent expression of fluorescent proteins represents a powerful biological tool for cell lineage, fate-mapping, and genetic analysis. Live tissue imaging has significantly improved with the development of far-red fluorescent proteins, with optimized spectral characteristics for in vivo applications. Here, we report the generation of the first transgenic mouse line expressing the far-red fluorescent protein Katushka, driven by the hybrid CAG promoter upon Cre-mediated recombination. After germ line or tissue-specific Cre-driven reporter activation, Katushka expression is strong and ubiquitous, without toxic effects, allowing fluorescence detection in fresh and fixed samples from all tissues examined. Moreover, fluorescence can be detected by in vivo noninvasive whole-body imaging when Katuhska is expressed exclusively in a specific cell population deep within the animal body such as pancreatic beta cells. Thus, this reporter model enables early, widespread, and sensitive in vivo detection of Cre activity and should provide a versatile tool for a wide spectrum of fluorescence and live-imaging applications. genesis 49:36-45, 2011. (C) 2010 Wiley-Liss, Inc.